A novel immunostimulating aspect of Lactobacillus gasseri: Induction of “Gasserokine” as chemoattractants for macrophages

Haruki Kitazawa, Tomohiko Ino, Yasushi Kawai, Takatoshi Itoh, Tadao Saito

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The chemotactic activity of the culture supernatants from 14 strains of Lactobacillus acidophilus and L. Gasseri was examined for murine macrophages. Significant macrophage chemotactic activity was observed in three strains of L. acidophilus and all strains of L. gasseri. The highest activity was observed in the supernatant (1131-sup) from 24-h cultures of L. gasseri JCM1131T. The chemotactic factor from 1131-sup, designated as “Gasserokine”, was purified by the C18 reverse phase and ion-exchange chromatography. The purity of Gasserokine was checked by HPLC with the reverse-phase mode. The chemotactic activity of Gasserokine was also observed for human monocytes. The macrophage chemotaxis induced by L. gasseri JCM1131T culture supernatants was discovered to be a new biological function exerted by probiotic lactic acid bacteria. Therefore, the activity is expected to be used for one of the functional parameters in the immunomodulating properties of probiotic lactic acid bacteria.

Original languageEnglish
Pages (from-to)29-38
Number of pages10
JournalInternational Journal of Food Microbiology
Volume77
Issue number1-2
DOIs
Publication statusPublished - 2002 Jul 25

Keywords

  • Chemotactic activity
  • Lactobacillus gasseri
  • Macrophage
  • Monocyte
  • Probiotics

ASJC Scopus subject areas

  • Food Science
  • Microbiology

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