A novel glycosylphosphatidyl inositol-anchored protein on human leukocytes: A possible role for regulation of neutrophil adherence and migration

Kichiya Suzuki, Tadashi Watanabe, Shin Ichi Sakurai, Kazuhisa Ohtake, Taroh Kinoshita, Akemi Araki, Teizo Fujita, Hiroshi Takei, Yuji Takeda, Yukiko Sato, Takao Yamashita, Yoshihiko Araki, Fujiro Sendo

Research output: Contribution to journalArticlepeer-review

81 Citations (Scopus)

Abstract

We report here a novel glycosylphosphatidyl-inositol (GPI)-anchored glycoprotein on human leukocytes. Treatment of neutrophils with a mAb (3H9) to this molecule sequentially up-regulates and down-regulates β2 integrin- dependent adhesion of these cells as well as their transendothelial migration in vitro. In addition, this mAb simultaneously modulates the avidity of β2 integrin for its ligand, iC3b, with kinetics similar to those observed in 3H9 modulation of neutrophil adherence. This mAb also induces β2 integrin- dependent cytoskeletal remodeling. This novel GPI-anchored protein (GPI-80) is highly homologous with Vanin-1, a recently reported GPI-anchored protein that is expressed on perivascular thymic stromal cells and is involved in thymus homing in mice. The finding that both GPI-80 and Vanin-1 are 40% homologous with human biotinidase suggests the existence of a biotinidase superfamily of molecules that may be involved in the regulation of leukocyte trafficking.

Original languageEnglish
Pages (from-to)4277-4284
Number of pages8
JournalJournal of Immunology
Volume162
Issue number7
Publication statusPublished - 1999 Apr 1
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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