TY - JOUR
T1 - A novel gene ″Niban″ upregulated in renal carcinogenesis
T2 - Cloning by the cDNA-amplified fragment length polymorphism approach
AU - Majima, Shuichi
AU - Kajino, Kazunori
AU - Fukuda, Tomokazu
AU - Otsuka, Fujio
AU - Hino, Okio
PY - 2000
Y1 - 2000
N2 - A modified AFLP (amplified fragment length polymorphism) method was employed to isolate genes differentially expressed in renal carcinogenesis of Tsc2 gene mutant (Eker) rats. One gene, selected for further investigation, was named ″Niban″ (″second″ in Japanese), because it is the second new gene to be found after Erc (expressed in renal carcinoma) in our laboratory. Importantly, ″Niban″ is well expressed even in small primary rat Eker renal tumors, more than in progressed cell lines, and is also expressed in human renal carcinoma cells, but not in normal human or rat kidneys. Chromosome assignment was to RNO 13 in the rat, and HSA 1. This ″Niban″ gene is a candidate as a marker for renal tumor, especially early-stage renal carcinogenesis.
AB - A modified AFLP (amplified fragment length polymorphism) method was employed to isolate genes differentially expressed in renal carcinogenesis of Tsc2 gene mutant (Eker) rats. One gene, selected for further investigation, was named ″Niban″ (″second″ in Japanese), because it is the second new gene to be found after Erc (expressed in renal carcinoma) in our laboratory. Importantly, ″Niban″ is well expressed even in small primary rat Eker renal tumors, more than in progressed cell lines, and is also expressed in human renal carcinoma cells, but not in normal human or rat kidneys. Chromosome assignment was to RNO 13 in the rat, and HSA 1. This ″Niban″ gene is a candidate as a marker for renal tumor, especially early-stage renal carcinogenesis.
KW - CDNA-AFLP
KW - Multistep renal carcinogenesis
KW - Tsc2 gene mutant (Eker) rats
KW - Tumor marker
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U2 - 10.1111/j.1349-7006.2000.tb01027.x
DO - 10.1111/j.1349-7006.2000.tb01027.x
M3 - Article
C2 - 11011112
AN - SCOPUS:0033623543
VL - 91
SP - 869
EP - 874
JO - Cancer Science
JF - Cancer Science
SN - 1347-9032
IS - 9
ER -
