A novel gene encoding a thrombin inhibitory protein in a cDNA library from Haemaphysalis longicornis salivary gland

Chie Nakajima, Saiki Imamura, Satoru Konnai, Shinji Yamada, Hideto Nishikado, Kazuhiko Ohashi, Misao Onuma

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

A novel thrombin inhibitory protein coding gene was identified from a cDNA library derived from salivary gland of partially-fed Haemaphysalis longicornis (hard tick). The gene encoded a 93-amino acid protein, designated chimadanin, which had a signal peptide sequence and was predicted to be a secretory protein. It showed no similarity to any other previously identified proteins or conserved domain sequences. The gene was expressed during blood feeding and suggested to be expressed mainly in the salivary gland. The predicted mature region of chimadanin was expressed in Escherichia coli and characteristics of the recombinant chimadanin were determined. The activated partial thromboplastin time and the prothrombin time in sheep plasma were significantly prolonged by chimadanin in a dose dependent manner. Amidolytic activity of thrombin was also inhibited by chimadanin in a dose dependent manner and it suggested that chimadanin was an anticoagulant with thrombin inhibitory activity. This newly identified thrombin inhibitor may play an important role in tick blood feeding.

Original languageEnglish
Pages (from-to)447-452
Number of pages6
JournalJournal of Veterinary Medical Science
Volume68
Issue number5
DOIs
Publication statusPublished - 2006

Keywords

  • Anticoagulant
  • Chimadanin
  • Haemaphysalis longicornis
  • Thrombin inhibitor
  • Tick

ASJC Scopus subject areas

  • veterinary(all)

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