Abstract
A novel thrombin inhibitory protein coding gene was identified from a cDNA library derived from salivary gland of partially-fed Haemaphysalis longicornis (hard tick). The gene encoded a 93-amino acid protein, designated chimadanin, which had a signal peptide sequence and was predicted to be a secretory protein. It showed no similarity to any other previously identified proteins or conserved domain sequences. The gene was expressed during blood feeding and suggested to be expressed mainly in the salivary gland. The predicted mature region of chimadanin was expressed in Escherichia coli and characteristics of the recombinant chimadanin were determined. The activated partial thromboplastin time and the prothrombin time in sheep plasma were significantly prolonged by chimadanin in a dose dependent manner. Amidolytic activity of thrombin was also inhibited by chimadanin in a dose dependent manner and it suggested that chimadanin was an anticoagulant with thrombin inhibitory activity. This newly identified thrombin inhibitor may play an important role in tick blood feeding.
| Original language | English |
|---|---|
| Pages (from-to) | 447-452 |
| Number of pages | 6 |
| Journal | Journal of Veterinary Medical Science |
| Volume | 68 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2006 |
| Externally published | Yes |
Keywords
- Anticoagulant
- Chimadanin
- Haemaphysalis longicornis
- Thrombin inhibitor
- Tick
ASJC Scopus subject areas
- veterinary(all)