A novel enzyme immunoassay for the determination of phosphatidylserine-specific phospholipase A1 in human serum samples

Kazuhiro Nakamura, Koji Igarashi, Ryunosuke Ohkawa, Naoya Saiki, Mika Nagasaki, Kansei Uno, Naoto Hayashi, Tetsuji Sawada, Kenichi Syukuya, Hiromitsu Yokota, Hiroyuki Arai, Hitoshi Ikeda, Junken Aoki, Yutaka Yatomi

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Background: The bioactive lipid lysophosphatidylserine (LPS) is postulated to induce important biological responses and to be produced by phosphatidylserine-specific phospholipase A1 (PS-PLA1). To evaluate the functional roles of LPS in vivo, a facile assay method for PS-PLA1 has been awaited. Methods: Recombinant human PS-PLA1 was produced using a baculovirus system, and anti-human PS-PLA1 monoclonal antibodies were generated. Two clones were then selected for a 2-site immunoassay. The resulting PS-PLA1 assay reagent was applied to a commercial automated immunoassay analyzer. Results: Satisfactory results were obtained for the within-run and between-run precision, interference, detection limit, and linearity of this PS-PLA1 assay. The mean±SD of the serum PS-PLA1 antigen concentration in the 191 healthy subjects was 33.8±16.6μg/l, and the central 95th percentile reference interval for the serum PS-PLA1 antigen concentration was 13.8-74.1μg/l. The concentration was significantly (p<0.001) higher among men (13.8-80.6μg/l) than among women (12.1-68.8μg/l). We did not find a correlation between PS-PLA1 and existing laboratory tests. Conclusions: The present PS-PLA1 assay method can be applied to clinical laboratory testing, and further studies are warranted to establish its clinical significance.

Original languageEnglish
Pages (from-to)1090-1094
Number of pages5
JournalClinica Chimica Acta
Volume411
Issue number1-16
DOIs
Publication statusPublished - 2010 Aug

Keywords

  • Enzyme immunoassay
  • Laboratory medicine
  • Lysophosphatidylserine
  • Phosphatidylserine-specific phospholipase A

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Fingerprint

Dive into the research topics of 'A novel enzyme immunoassay for the determination of phosphatidylserine-specific phospholipase A<sub>1</sub> in human serum samples'. Together they form a unique fingerprint.

Cite this