A novel cis-acting element regulates HES-1 gene expression in P19 embryonal carcinoma cells treated with retinoic acid

Nobunao Wakabayashi, Ryoichiro Kageyama, Toshiyuki Habu, Toshihide Doi, Takashi Morita, Masami Nozaki, Masayuki Yamamoto, Yoshitake Nishimune

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The regulatory mechanisms of mammalian hairy and Enhancer of split homologue-1 (HES-1) genes were examined in mouse P19 embryonic carcinoma cells (P19 cells). Undifferentiated P19 stem cells expressed a basal level of the HES-1 gene, whereas the expression of this gene was increased upon induction of the cells to a neural cell lineage using retinoic acid (RA). Reporter co-transfection analysis identified an activating region within the upstream promoter region of HES-1 from nucleotides -201 to -172. This activating region, called activating region X (ARX), shows a high GC content and contains both an AP-2 binding motif and a CCAAT box. An electrophoretic mobility shift assay using nuclear proteins extracted from P19 cells showed that ARX forms a specific DNA-protein complex. Importantly, ARX-dependent transcription, as well as ARX-binding activity, was significantly increased in P19 cells treated with RA. These results indicare that ARX transduces signals that up-regulate HES-1 gene expression in response to RA-treatment. Thus, a novel cis-acting element involved in HES-1 gene regulation that plays a role in RA-induced neural differentiation of P19 cells has been identified.

Original languageEnglish
Pages (from-to)1087-1095
Number of pages9
JournalJournal of biochemistry
Volume128
Issue number6
DOIs
Publication statusPublished - 2000 Jan 1
Externally publishedYes

Keywords

  • Electrophoretic mobility shift assay
  • Neural differentiation
  • P19 embryonic carcinoma cells
  • Retinoic acid
  • Transcription factor

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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