A novel assay for detection and quantification of C-mannosyl tryptophan in normal or diabetic mice

Sho Sakurai, Yoko Inai, Shiho Minakata, Shino Manabe, Yukishige Ito, Yoshito Ihara

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8 Citations (Scopus)


C-Mannosyl tryptophan (C-Man-Trp) is a unique molecule in that an α-mannose is connected to the indole C2 carbon atom of a Trp residue via C-glycosidic linkage. Although serum C-Man-Trp may be a novel biomarker of renal function in humans, the biological significance of C-Man-Trp has yet to be fully investigated. In this study, a novel assay system for C-Man-Trp was established using hydrophilic-interaction liquid chromatography, followed by detecting the fluorescence intensity or mass abundance of C-Man-Trp. Using this system, we systematically assessed the amount of free monomeric C-Man-Trp in different tissues of mice. The tissue level of C-Man-Trp was high, especially in the ovaries and uterus. Other organs with high levels of C-Man-Trp included the brain, spleen, lungs, bladder, and testes. The level was low in skeletal muscle. We also investigated whether the tissue level of C-Man-Trp is affected in diabetes. In KK-Ay diabetic mice, the level of urinary C-Man-Trp excretion was increased, and the tissue levels of C-Man-Trp were decreased in the liver but increased in the kidney. These results demonstrate that C-Man-Trp is differentially distributed in numerous tissues and organs in mice, and the levels are altered by disordered carbohydrate metabolism such as diabetes.

Original languageEnglish
Article number4675
JournalScientific reports
Issue number1
Publication statusPublished - 2019 Dec 1
Externally publishedYes

ASJC Scopus subject areas

  • General


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