A novel approach to collecting satellite cells from adult skeletal muscles on the basis of their stress tolerance

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5 Citations (Scopus)

Abstract

cell surface marker is not well determined. Satellite cells, which are skeletal muscle stem cells, have the ability to regenerate damaged muscles and are expected to be applicable for treatment of muscle degeneration. Although the transcription factor Pax7 is a known specific marker of satellite cells, it is not located on the cell surface and therefore flow cytometry is not directly applicable. In the present study, we turned our attention to the stress tolerance of adult stem cells, and we propose long-term trypsin incubation (LTT) as a novel approach to collecting satellite cells from mouse and human skeletal muscles. LTT led to a remarkable increase in the ratio of Pax7(+) cells that retain normal myogenic stem cell function. In particular, human Pax7(+) cells made up approximately 30% of primary cultured cells, whereas after LTT, the ratio of Pax7(+) cells increased up to ̃80%, and the ratio of Pax7(+) and/or MyoD(+) myogenic cells increased to ̃95%. Once transplanted, LTT-treated cells contributed to subsequent muscle regeneration following repetitive muscle damage without additional cell transplantation. The stress tolerance of Pax7(+) cells is related to heat shock protein 27 and αB-crystallin, members of the small heat shock protein family. This approach, based on the stress resistance of adult stem cells, is a safe and inexpensive method of efficiently collecting human satellite cells and may also be used for collecting other tissue stem cells whose surface marker is unknown.

Original languageEnglish
Pages (from-to)488-498
Number of pages11
JournalStem Cells Translational Medicine
Volume2
Issue number7
DOIs
Publication statusPublished - 2013 Jul

Keywords

  • Adult stem cells
  • Muscle stem cells
  • Somatic cell therapy
  • Tissue-specific stem cells

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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