A novel Acyl-CoA synthetase, ACS5, expressed in intestinal epithelial cells and proliferating preadipocytes

Eisaku Oikawa, Hiroaki Iijima, Takashi Suzuki, Hironobu Sasano, Hiroyuki Sato, Akihisa Kamataki, Hiroshi Nagura, Man Jong Kang, Takahiro Fujino, Hiroyuki Suzuki, Tokuo T. Yamamoto

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111 Citations (Scopus)

Abstract

We report here the identification, characterization, and expression of a novel rat acyl-CoA synthetase (ACS) designated as ACS5. ACS5 consists of 683 amino acids and is approximately 60% identical to the previously characterized ACS1 and ACS2. ACS5 was overproduced in Escherichia coli cells and then purified to near homogeneity. The purified enzyme utilized a wide range of saturated fatty acids similar to those utilized by ACS1 and ACS5., but differed in its preference for C16-C18 unsaturated fatty acids. Northern blot analysis revealed that ACS5 mRNA is present most abundantly in the small intestine, and to a much lesser extent in the lung, liver, adrenal gland, adipose tissue, and kidney. In situ hybridization of rat ileum revealed abundant accumulation of ACS5. transcripts in foveolar epithelial cells. The hepatic level of ACS5 mRNA was significantly increased by refeeding a fat-free high sucrose diet and reduced by fasting or refeeding a high cholesterol diet, whereas that in the small intestine was not significantly altered by various dietary conditions. In contrast to the absence of ACS1 mRNA in undifferentiated 3T3-L1 preadipocytes, ACS5 mRNA was present in proliferating 3T3-L1 preadipocytes and its level remained unaltered during differentiation, suggesting that ACS5 may provide the acyl-CoA utilized for the synthesis of cellular lipids in proliferating preadipocytes.

Original languageEnglish
Pages (from-to)679-685
Number of pages7
JournalJournal of biochemistry
Volume124
Issue number3
DOIs
Publication statusPublished - 1998 Sep

Keywords

  • Acyl-CoA synthetase
  • Dietary regulation
  • Intestinal epithelial cell
  • Lipogenesis
  • Proliferation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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    Oikawa, E., Iijima, H., Suzuki, T., Sasano, H., Sato, H., Kamataki, A., Nagura, H., Kang, M. J., Fujino, T., Suzuki, H., & Yamamoto, T. T. (1998). A novel Acyl-CoA synthetase, ACS5, expressed in intestinal epithelial cells and proliferating preadipocytes. Journal of biochemistry, 124(3), 679-685. https://doi.org/10.1093/oxfordjournals.jbchem.a022165