A Non-invasive Imageable GFP-expressing Mouse Model of Orthotopic Human Bladder Cancer

Yu Sun, Hiroto Nishino, Ming Zhao, Kentaro Miyake, Norihiko Sugisawa, Jun Yamamoto, Yoshihiko Tashiro, Sachiko Inubushi, Kazuyuki Hamada, Guangwei Zhu, Hyein Lim, Robert M. Hoffman

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Background/Aim: A more realistic mouse model of bladder cancer is necessary to develop effective drugs for the disease. Tumor models enhanced by bright fluorescent-reporter genes to follow the disease in real-time would enhance the ability to accurately predict the efficacy of various therapeutics on this particularly-malignant human cancer. Materials and Methods: A highly-fluorescent green fluorescent protein (GFP)expressing bladder cancer model was orthotopically established in nude mice using the UM-UC-3 human bladder-cancer cell line (UM-UC-3-GFP). Fragments from a subcutaneous tumor of UM-UC-3-GFP were surgically implanted into the nude mouse bladder. Non-invasive and intra-vital fluorescence imaging was obtained with a simple imaging box. Results: The GFP-expressing orthotopic bladder tumor was imaged in real-time non-invasively as well as intra-vitally, with the two methods correlating at r=0.99. Conclusion: This is the first noninvasive-fluorescence-imaging orthotopic model of bladder cancer and can be used for rapidly screening novel effective agents for this recalcitrant disease.

Original languageEnglish
Pages (from-to)3225-3231
Number of pages7
JournalIn Vivo
Volume34
Issue number6
DOIs
Publication statusPublished - 2020 Nov
Externally publishedYes

Keywords

  • Bladder cancer
  • GFP
  • Imaging
  • Non-invasive
  • Nude mice
  • Orthotopic

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

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