A non-invasive biomonitoring method for assessing levels of urinary pyrethroid metabolites in diapered children by gas chromatography-mass spectrometry

Shun Saito, Jun Ueyama, Takaaki Kondo, Isao Saito, Eiji Shibata, Masahiro Gotoh, Hiroshi Nomura, Shinya Wakusawa, Kunihiko Nakai, Michihiro Kamijima

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

The aim of this study was to develop a method for quantitative measurement of urinary metabolites of pyrethroid (PYR) insecticides, trans- chrysanthemumdicarboxylic acid (CDCA) and 3-phenoxybenzoic acid (3-PBA), extracted from disposable diapers. This study was approved by the university ethics committees, and informed consent was obtained from all the parents for their children and from adult volunteers. After extraction of PYR metabolites in the absorber of diapers with 5 ml acetone, the metabolites in the eluents were extracted with tert-butyl methyl ether, derivatized with 1,1,1,3,3,3- hexafluoroisopropanol and analyzed by gas chromatography-mass spectrometry. The limits of quantitation (LOQs) were 0.55 μg/l for CDCA and 0.09 μg/l for 3-PBA in 2 ml urine extracted from diapers. Within-series and between-day precisions were <14% (CV%) over the concentration range of metabolites from 0.4 to 20.4 μg/l urine. When concentrations of each metabolite were measured with the developed method after pouring 2 ml urine, which was obtained from adults both in a general population and pest control operators, on diapers, good correlations were shown between the measured results and the concentrations measured directly for the respective urine with the conventional method (Spearman's rank correlation coefficient 0.889 for CDCA and 0.989 for 3-PBA; n=27-28). The developed method would be applicable to epidemiological studies.

Original languageEnglish
Pages (from-to)200-207
Number of pages8
JournalJournal of Exposure Science and Environmental Epidemiology
Volume24
Issue number2
DOIs
Publication statusPublished - 2014 Mar

Keywords

  • acetone extraction
  • children
  • disposable diaper
  • gas chromatography-mass spectrometry
  • pyrethroid insecticide
  • urine

ASJC Scopus subject areas

  • Epidemiology
  • Toxicology
  • Pollution
  • Public Health, Environmental and Occupational Health

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