A new strategy for site-specific alkylation of DNA using oligonucleotides containing an abasic site and alkylating probes

Norihiro Sato, Genichiro Tsuji, Yoshihiro Sasaki, Akira Usami, Takuma Moki, Kazumitsu Onizuka, Ken Yamada, Fumi Nagatsugi

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Selective chemical reactions with DNA, such as its labelling, are very useful in many applications. In this paper, we discuss a new strategy for the selective alkylation of DNA using an oligonucleotide containing an abasic site and alkylating probes. We designed three probes consisting of 2-AVP as a reactive moiety and three kinds of binding moiety with high affinity to duplex DNA. Among these probes, Hoechst-AVP probe exhibited high selectivity and efficient reactivity to thymine bases at the site opposite an abasic site in DNA. Our method is potentially useful for inducing site-directed reactions aimed at inhibiting polymerase reactions.

Original languageEnglish
Pages (from-to)14885-14888
Number of pages4
JournalChemical Communications
Volume51
Issue number80
DOIs
Publication statusPublished - 2015 Aug 19

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Fingerprint Dive into the research topics of 'A new strategy for site-specific alkylation of DNA using oligonucleotides containing an abasic site and alkylating probes'. Together they form a unique fingerprint.

Cite this