A New Screening Method for the Selection of Lactobacillus acidophilus Group Lactic Acid Bacteria with High Adhesion to Human Colonic Mucosa

Noritoshi Takahashi, Tadao Saito, Shyuichi Ohwada, Hiroyoshi Ota, Honoh Hashiba, Takatoshi Itoh

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

A hemagglutination (HA) assay was done for the screening of lectin-like components in surface layer protein (SLP) from Lactobacillus (L.) acidophilus A group strains. The new screening method, using polystyrene beads coated with rat-colonic mucin (RCM), which combines sugar chains similar to those of human colonic mucin, was also done. The results showed that the HA assay was not a good indicator for selecting strains having high adhesion to the human intestinal tract. The SLPs from 3 strains that strongly bound to RCM also bound well to carbohydrate portions of Carnoy’s-fixed human colonic mucous layer. These results suggest that this method is a new promising screening technique for the L. acidophilus strains having high adhesion to the human intestinal tract.

Original languageEnglish
Pages (from-to)1434-1438
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume60
Issue number9
DOIs
Publication statusPublished - 1996 Jan

Keywords

  • Hemagglutination
  • Human colonic mucosa
  • Lactobacillus acidophilus
  • Lectin
  • Rat-colonic mucin

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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