A new primer set to amplify the mitochondrial cytochrome C oxidase subunit I (COI) gene in the DHA-rich microalgae, the genus Aurantiochytrium

Goh Nishitani, Masaki Yoshida

    Research output: Contribution to journalArticlepeer-review

    Abstract

    This study was performed in order to develop a primer set for mitochondrial cytochrome c oxidase subunit I (COI) in the DHA-rich microalgae of the genus Aurantiochytrium. The performance of the primer set was tested using 12 Aurantiochytrium strains and other thraustochytrid species. There were no genetic polymorphisms in the mitochondrial sequences from the Aurantiochytrium strains, in contrast to the nuclear 18S rRNA gene sequence. This newly developed primer set amplified sequences from Aurantiochytrium and closely related genera, and may be useful for species identification and clarifying the genetic diversity of Aurantiochytrium in the field.

    Original languageEnglish
    Pages (from-to)227-229
    Number of pages3
    JournalMicrobes and environments
    Volume33
    Issue number2
    DOIs
    Publication statusPublished - 2018

    Keywords

    • Aurantiochytrium
    • COI
    • Mitochondrial primer

    ASJC Scopus subject areas

    • Ecology, Evolution, Behavior and Systematics
    • Soil Science
    • Plant Science

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