A new primer set to amplify the mitochondrial cytochrome C oxidase subunit I (COI) gene in the DHA-rich microalgae, the genus Aurantiochytrium

Goh Nishitani, Masaki Yoshida

Research output: Contribution to journalArticlepeer-review

Abstract

This study was performed in order to develop a primer set for mitochondrial cytochrome c oxidase subunit I (COI) in the DHA-rich microalgae of the genus Aurantiochytrium. The performance of the primer set was tested using 12 Aurantiochytrium strains and other thraustochytrid species. There were no genetic polymorphisms in the mitochondrial sequences from the Aurantiochytrium strains, in contrast to the nuclear 18S rRNA gene sequence. This newly developed primer set amplified sequences from Aurantiochytrium and closely related genera, and may be useful for species identification and clarifying the genetic diversity of Aurantiochytrium in the field.

Original languageEnglish
Pages (from-to)227-229
Number of pages3
JournalMicrobes and environments
Volume33
Issue number2
DOIs
Publication statusPublished - 2018

Keywords

  • Aurantiochytrium
  • COI
  • Mitochondrial primer

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science
  • Plant Science

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