A new low density lipoprotein receptor related protein, LRP5, is expressed in hepatocytes and adrenal cortex, and recognizes apolipoprotein E

Dong Ho Kim, Yousuke Inagaki, Takashi Suzuki, Ryoichi X. Ioka, Shiroh Z. Yoshioka, Kenta Magoori, Man Jong Kang, Yuko Cho, Akira Z. Nakano, Qiong Liu, Takahiro Fujino, Hiroyuki Suzuki, Hironobu Sasano, Tokuo T. Yamamoto

Research output: Contribution to journalArticlepeer-review

96 Citations (Scopus)

Abstract

The isolation and characterization of rabbit and human cDNAs revealed a new low density lipoprotein receptor (LDLR)-related protein (LRP) designated as LRP5. Human LRP5 cDNA encodes a 1616-amino acid type I membrane-like protein with three ligand binding repeats in its extracellular region. LDLR-deficient cells transduced by recombinant adenovirus containing human LRP5 exhibited increased binding of apolipoprotein E (apoE)-enriched β-migrating very low density lipoprotein. Northern blotting and in situ hybridization revealed a high level of LRP5 expression in hepatocytes and the adrenal gland cortex. In LDLR-deficient Watanabe heritable hyperlipidemic rabbits, LRP5 mRNA was increased in the liver and accumulated in cholesterol-laden foam cells of atherosclerotic lesions.

Original languageEnglish
Pages (from-to)1072-1076
Number of pages5
JournalJournal of biochemistry
Volume124
Issue number6
DOIs
Publication statusPublished - 1998

Keywords

  • LDL receptor family
  • LDL receptor-related protein
  • Lipoprotein
  • apoE

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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