A new lead chemical for strigolactone biosynthesis inhibitors

Shinsaku Ito, Nobutaka Kitahata, Mikihisa Umehara, Atsushi Hanada, Atsutaka Kato, Kotomi Ueno, Kiyoshi Mashiguchi, Junko Kyozuka, Koichi Yoneyama, Shinjiro Yamaguchi, Tadao Asami

    Research output: Contribution to journalArticlepeer-review

    33 Citations (Scopus)


    Several triazole-containing chemicals have previously been shown to act as efficient inhibitors of cytochrome P450 monooxygenases. To discover a strigolactone biosynthesis inhibitor, we screened a chemical library of triazole derivatives to find chemicals that induce tiller bud outgrowth of rice seedlings. We discovered a triazole-type chemical, TIS13 [2,2-dimethyl-7- phenoxy-4-(1H-1,2,4-triazol-1-yl)heptan-3-ol], which induced outgrowth of second tiller buds of wild-type seedlings, as observed for non-treated strigolactone-deficient d10 mutant seedlings. TIS13 treatment reduced strigolactone levels in both roots and root exudates in a concentration- dependent manner. Co-application of GR24, a synthetic strigolactone, with TIS13 canceled the TIS13-induced tiller bud outgrowth. Taken together, these results indicate that TIS13 inhibits strigolactone biosynthesis in rice seedlings. We propose that TIS13 is a new lead compound for the development of specific strigolactone biosynthesis inhibitors.

    Original languageEnglish
    Pages (from-to)1143-1150
    Number of pages8
    JournalPlant and Cell Physiology
    Issue number7
    Publication statusPublished - 2010 Jul


    • Chemical library
    • Inhibitor
    • P450
    • Rice
    • Strigolactone
    • Triazole

    ASJC Scopus subject areas

    • Physiology
    • Plant Science
    • Cell Biology


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