A new hexasaccharide chain isolated from bovine colostrum κ-casein taken at the time of parturition

Tadao Saito, Takatoshi Itoh, Susumu Adachi, Tateo Suzuki, Taichi Usui

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

The carbohydrate units linked to caseinoglycopeptide from colostrum taken 30 min after parturition were released as reduced oligosaccharides by alkaline borohydride treatment, and separated into four acidic oligosacchariditols (a hexa- (11.0%), penta- (38.5%), tetra- (35.5%) and a trisacchariditol (15.0%)). Structural studies showed that the hexasacchariditol was a new structure and had the chemical structure: NeuNAc-α-2-3-Gal-β-1-3-[NeuNAc-α-2-3-Gal-β-1-4-GlcNAc-β-1-6-]-N-acetylgalactosaminitol(GalNAcItol). The other three oligosacchariditols were shown to be NeuNAc-α-2-3-Gal-β-1-3-[Gal-β-1-4-GlcNac- β-1-6-]-GalNAcItol, NeuNAc-α-2-3-Gal-β-1-3-[NeuNAc-α-2-6-]-GalNAcItol and NeuNAc-α-2-3-Gal-β-1-3- GalNAcItol, which were identical with those found previously in colostrum κ-casein taken 6 h after parturition.

Original languageEnglish
Pages (from-to)309-317
Number of pages9
JournalBBA - General Subjects
Volume719
Issue number2
DOIs
Publication statusPublished - 1982 Nov 24

Keywords

  • (Bovine colostrum)
  • Carbohydrate sequence
  • Hexasacchariditol
  • Parturition
  • κ-Casein

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'A new hexasaccharide chain isolated from bovine colostrum κ-casein taken at the time of parturition'. Together they form a unique fingerprint.

Cite this