A new fluorescence band F689 in photosystem II revealed by picosecond analysis at 4-77 K: Function of two terminal energy sinks F689 and F695 in PS II

Masayuki Komura, Yutaka Shibata, Shigeru Itoh

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

We performed picosecond time-resolved fluorescence spectroscopy in spinach photosystem II (PS II) particles at 4, 40, and 77 K and identified a new fluorescence band, F689. F689 was identified in addition to the well-known F685 and F695 bands in both analyses of decay-associated spectra and global Gaussian deconvolution of time-resolved spectra. Its fast decay suggests the energy transfer directly from F689 to the reaction center chlorophyll P680. The contribution of F689, which increases only at low temperature, explains the unusually broad and variable bandwidth of F695 at low temperature. Global analysis revealed the three types of excitation energy transfer/dissipation processes: (1) energy transfer from the peripheral antenna to the three core antenna bands F685, F689, and F695 with time constants of 29 and 171 ps at 77 and 4 K, respectively; (2) between the three core bands (0.18 and 0.82 ns); and (3) the decays of F689 (0.69 and 3.02 ns) and F695 (2.18 and 4.37 ns). The retardations of these energy transfer rates and the slow F689 decay rate produced the strong blue shift of the PS II fluorescence upon the cooling below 77 K.

Original languageEnglish
Pages (from-to)1657-1668
Number of pages12
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1757
Issue number12
DOIs
Publication statusPublished - 2006 Dec
Externally publishedYes

Keywords

  • Chlorophyll fluorescence
  • Excitation energy transfer
  • Global analysis
  • Photosystem II
  • Temperature dependency
  • Time-resolved fluorescence spectroscopy

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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