A new family of endogenous "big" met-enkephalins from bovine adrenal medulla: purification and structure of docosa- (BAM-22P) and eicosapeptide (BAM-20P) with very potent opiate activity

Kensaku Mizuno, Naoto Minamino, Kenji Kangawa, Hisayuki Matsuo

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

Two novel "big" Met-enkephalins,6a docosa-(BAM-22P) and an eicosapeptide(BAM-20P), both of which yield [Arg6]-Met-enkephalin upon trypsinization, have been purified from the bovine adrenal medulla. The complete sequence of BAM-22P has been established, while BAM-20P has also been proved to be a C-terminal shortened peptide of BAM-22P. Both peptides contain BAM-12P sequence, that previously determined, at their N-terminals with progressive C-terminal extension. The identification of BAM-22P and -20P along with BAM-12P in the tissue presented a new family of "big" Met-enkephalins indicative of the unique feature of enkephalin biosynthesis. In addition, BAM-22P and -20P have extraordinarily potent opiate activity in guinea-pig ileum assay.

Original languageEnglish
Pages (from-to)1283-1290
Number of pages8
JournalBiochemical and biophysical research communications
Volume97
Issue number4
DOIs
Publication statusPublished - 1980 Dec 31
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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