A new endogenous opioid peptide from bovine adrenal medulla: Isolation and amino acid sequence of a dodecapeptide (BAM-12P)

Kensaku Mizuno, Naoto Minamino, Kenji Kangawa, Hisayuki Matsuo

Research output: Contribution to journalArticlepeer-review

67 Citations (Scopus)

Abstract

A new dodecapeptide, designated BAM-12P, which released an N-terminal [Arg6]-Met-enkephalin by trypsinization, has been isolated from bovine adrenal medulla in a pure state by the aid of a newly developed radioimmunoassay utilizing antiserum against [Arg6]-Leu-enkephalin, which shows 5% cross-reactivity with [Arg6]-Met-enkephalin. Amino acid sequence of the peptide has been established to be: Tyr-Gly-Gly-Phe-Met-Arg-Arg-Val-Gly-Arg-Pro-Glu. On the basis of its structure, the dodecapeptide is considered to be a novel pro-Met-enkephalin, unrelated to β-lipotropin.

Original languageEnglish
Pages (from-to)1482-1488
Number of pages7
JournalTopics in Catalysis
Volume95
Issue number4
DOIs
Publication statusPublished - 1980 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Biophysics
  • Molecular Biology

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