A new approach to inhibiting astrocytic IP3-induced intracellular calcium increase in an astrocyte-neuron co-culture system

Yasunori Saheki, Sheng Tian Li, Masayuki Matsushita, Yu Mei Wu, Wei Hua Cai, Fan Yan Wei, Yun Fei Lu, Akiyoshi Moriwaki, Kazuhito Tomizawa, Hideki Matsui

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Astrocytes exhibit dynamic Ca2+ mobilization, such as Ca 2+ wave and Ca2+ oscillation, via an inositol 1,4,5-triphosphate-induced Ca2+ release (IICR)-dependent mechanism. The physiological functions of astrocytic Ca2+ mobilization, however, are poorly understood. To investigate this issue, we created a plasmid encoding an enhanced green fluorescent protein-tagged inositol 1,4,5-triphosphate absorbent protein and expressed it in cultured astrocytes. Expression of this protein inhibited both IICR and the Ca2+ wave in cultured astrocytes. By combining this method to the single cell electroporation technique, we were able to inhibit IICR specifically in astrocytes in an astrocyte-neuron co-culture system. Our approach provides a useful tool for direct examination of the physiological role of astrocytic Ca2+ signaling on neuronal function.

Original languageEnglish
Pages (from-to)196-201
Number of pages6
JournalBrain research
Volume1055
Issue number1-2
DOIs
Publication statusPublished - 2005 Sep 7
Externally publishedYes

Keywords

  • Astrocyte
  • Calcium wave
  • IP3
  • IP3 scavenger
  • Single cell electroporation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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  • Cite this

    Saheki, Y., Li, S. T., Matsushita, M., Wu, Y. M., Cai, W. H., Wei, F. Y., Lu, Y. F., Moriwaki, A., Tomizawa, K., & Matsui, H. (2005). A new approach to inhibiting astrocytic IP3-induced intracellular calcium increase in an astrocyte-neuron co-culture system. Brain research, 1055(1-2), 196-201. https://doi.org/10.1016/j.brainres.2005.06.056