A new and suitable reconstructed system for NADPH-dependent microsomal lipid peroxidation

Hisanori Minakami, Hiroyuki Arai, Minoru Nakano, Katsuaki Sugioka, Shingo Suzuki, Akemi Sotomatsu

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

In order to evaluate the O2- participation in NADPH-dependent microsomal lipid peroxidation, we used reconstructed system which contained detergent-solubilized NADPH-dependent cytochrome P-450 reductase, cytochrome P-450, phospholipid liposomes, NADPH and Fe3+-ADP. Lipid peroxidation, monitored by the formation of thiobarbituric acid-reactive substance, was increased with increasing concentration of detergent-solubilized NADPH cytochrome P-450 reductase, cytochrome P-450 or Fe3+-ADP. Cytochrome P-450-dependent lipid peroxidation was parallel to O2- generation monitored by chemiluminescence probe with 2-methyl-6-(p-methoxyphenol)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one. Lipid peroxidation was significantly inhibited by superoxide dismutase, but not by catalase or sodium benzoate. The reconstructed system herein described is considered to be very close to NADPH-dependent microsomal lipid peroxidation system.

Original languageEnglish
Pages (from-to)973-978
Number of pages6
JournalBiochemical and biophysical research communications
Volume153
Issue number3
DOIs
Publication statusPublished - 1988 Jun 30
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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