A New and Specific Enzyme-Linked Immunosorbent Assay for the Detection of C3 Nephritic Factor

Jin Seino; Kazuo Fukuda; Yasumichi Kinoshita; Katsuhiko Sudo; Ikuo Horigome; Hiroshi Sato; Takao Saito; Takashi Furuyama; Kaoru Yoshinaga

doi:10.1620/tjem.147.111

A New and Specific Enzyme-Linked Immunosorbent Assay for the Detection of C3 Nephritic Factor

Jin Seino, Kazuo Fukuda, Yasumichi Kinoshita, Katsuhiko Sudo, Ikuo Horigome, Hiroshi Sato, Takao Saito, Takashi Furuyama, Kaoru Yoshinaga

PHA - Pharmacy

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

C3 nephritic factor (C3 NeF) was measured by assessing its capacity to form complex with C3 and ? using an enzyme-linked immunosorbent assay (ELISA). Incubation of C3 NeF with normal human serum in the presence of MgEGTA resulted in a dose-dependent increase of C3-B-IgG complex. No complex was formed in EDTA. The C3 NeF titer estimated in this way was in good accordance with those reported previously by other indirect methods. C3 NeF ; C3-B-IgG complex ; ELISA.

Original language	English
Pages (from-to)	111-112
Number of pages	2
Journal	the tohoku journal of experimental medicine
Volume	147
Issue number	1
DOIs	https://doi.org/10.1620/tjem.147.111
Publication status	Published - 1985

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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A New and Specific Enzyme-Linked Immunosorbent Assay for the Detection of C3 Nephritic Factor. / Seino, Jin; Fukuda, Kazuo; Kinoshita, Yasumichi; Sudo, Katsuhiko; Horigome, Ikuo; Sato, Hiroshi; Saito, Takao; Furuyama, Takashi; Yoshinaga, Kaoru.

In: the tohoku journal of experimental medicine, Vol. 147, No. 1, 1985, p. 111-112.

Research output: Contribution to journal › Article › peer-review

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AU - Horigome, Ikuo

AU - Sato, Hiroshi

AU - Saito, Takao

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