TY - JOUR
T1 - A New and Specific Enzyme-Linked Immunosorbent Assay for the Detection of C3 Nephritic Factor
AU - Seino, Jin
AU - Fukuda, Kazuo
AU - Kinoshita, Yasumichi
AU - Sudo, Katsuhiko
AU - Horigome, Ikuo
AU - Sato, Hiroshi
AU - Saito, Takao
AU - Furuyama, Takashi
AU - Yoshinaga, Kaoru
PY - 1985
Y1 - 1985
N2 - C3 nephritic factor (C3 NeF) was measured by assessing its capacity to form complex with C3 and ? using an enzyme-linked immunosorbent assay (ELISA). Incubation of C3 NeF with normal human serum in the presence of MgEGTA resulted in a dose-dependent increase of C3-B-IgG complex. No complex was formed in EDTA. The C3 NeF titer estimated in this way was in good accordance with those reported previously by other indirect methods. C3 NeF ; C3-B-IgG complex ; ELISA.
AB - C3 nephritic factor (C3 NeF) was measured by assessing its capacity to form complex with C3 and ? using an enzyme-linked immunosorbent assay (ELISA). Incubation of C3 NeF with normal human serum in the presence of MgEGTA resulted in a dose-dependent increase of C3-B-IgG complex. No complex was formed in EDTA. The C3 NeF titer estimated in this way was in good accordance with those reported previously by other indirect methods. C3 NeF ; C3-B-IgG complex ; ELISA.
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U2 - 10.1620/tjem.147.111
DO - 10.1620/tjem.147.111
M3 - Article
C2 - 3934791
AN - SCOPUS:0022252155
VL - 147
SP - 111
EP - 112
JO - Tohoku Journal of Experimental Medicine
JF - Tohoku Journal of Experimental Medicine
SN - 0040-8727
IS - 1
ER -