Abstract
C3 nephritic factor (C3 NeF) was measured by assessing its capacity to form complex with C3 and ? using an enzyme-linked immunosorbent assay (ELISA). Incubation of C3 NeF with normal human serum in the presence of MgEGTA resulted in a dose-dependent increase of C3-B-IgG complex. No complex was formed in EDTA. The C3 NeF titer estimated in this way was in good accordance with those reported previously by other indirect methods. C3 NeF ; C3-B-IgG complex ; ELISA.
| Original language | English |
|---|---|
| Pages (from-to) | 111-112 |
| Number of pages | 2 |
| Journal | the tohoku journal of experimental medicine |
| Volume | 147 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1985 |
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
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Seino, J., Fukuda, K., Kinoshita, Y., Sudo, K., Horigome, I., Sato, H., Saito, T., Furuyama, T., & Yoshinaga, K. (1985). A New and Specific Enzyme-Linked Immunosorbent Assay for the Detection of C3 Nephritic Factor. the tohoku journal of experimental medicine, 147(1), 111-112. https://doi.org/10.1620/tjem.147.111