A New and Specific Enzyme-Linked Immunosorbent Assay for the Detection of C3 Nephritic Factor

Jin Seino, Kazuo Fukuda, Yasumichi Kinoshita, Katsuhiko Sudo, Ikuo Horigome, Hiroshi Sato, Takao Saito, Takashi Furuyama, Kaoru Yoshinaga

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

C3 nephritic factor (C3 NeF) was measured by assessing its capacity to form complex with C3 and ? using an enzyme-linked immunosorbent assay (ELISA). Incubation of C3 NeF with normal human serum in the presence of MgEGTA resulted in a dose-dependent increase of C3-B-IgG complex. No complex was formed in EDTA. The C3 NeF titer estimated in this way was in good accordance with those reported previously by other indirect methods. C3 NeF ; C3-B-IgG complex ; ELISA.

Original languageEnglish
Pages (from-to)111-112
Number of pages2
Journalthe tohoku journal of experimental medicine
Volume147
Issue number1
DOIs
Publication statusPublished - 1985

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'A New and Specific Enzyme-Linked Immunosorbent Assay for the Detection of C3 Nephritic Factor'. Together they form a unique fingerprint.

  • Cite this

    Seino, J., Fukuda, K., Kinoshita, Y., Sudo, K., Horigome, I., Sato, H., Saito, T., Furuyama, T., & Yoshinaga, K. (1985). A New and Specific Enzyme-Linked Immunosorbent Assay for the Detection of C3 Nephritic Factor. the tohoku journal of experimental medicine, 147(1), 111-112. https://doi.org/10.1620/tjem.147.111