A nano-needle interface self-assembled by using cell migration for recording intracellular activity: Nano-needle durability

Takayuki Hoshino, Tomohiro Konno, Kazuhiko Ishihara, Keisuke Morishima

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

This paper proposes a nano-needle intracellular interface which was assembled by using a cell's own locomotion force. This interface was designed for large scale intracellular recording for development of an artificial synaptic device. We previously demonstrated a method to insert a nanostructure into a living cell using cell locomotion. To achieve an intracellular nanoelectrode, we employed such cell migration on an extracellular matrix as the driving force to insert a nanoelectrode into a plasma membrane. In this study, we describe intracellular nanoelectrodes inserted by the cell migration force which are used to construct intracellular recording devices. Each microwell had a low cell binding glass plate which had cellular adhesive molecules, and there was an electrically conductive tungsten nanoneedle about 190 nm in diameter on the bottom of the microwell. Cells can adhere only in the microwell; thus they are pulled down by their own body and are inserted into the nanoneedle by their own migration force. Then, the living cellular activity can be measured from the electrically conductive nanoneedle. Using this concept, we attempted to achieve a long-lasting intracellular multi channel interface between living cells and artificial implements without making a precise mechanical alignment and without an invasive procedure. This report explains the fabrication and electrical characteristics of the interface for development of the physical part of the cell-machine interface.

Original languageEnglish
Title of host publicationProceedings of the 2nd Biennial IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, BioRob 2008
Pages506-510
Number of pages5
DOIs
Publication statusPublished - 2008 Dec 1
Externally publishedYes
Event2nd Biennial IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, BioRob 2008 - Scottsdale, AZ, United States
Duration: 2008 Oct 192008 Oct 22

Publication series

NameProceedings of the 2nd Biennial IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, BioRob 2008

Other

Other2nd Biennial IEEE/RAS-EMBS International Conference on Biomedical Robotics and Biomechatronics, BioRob 2008
CountryUnited States
CityScottsdale, AZ
Period08/10/1908/10/22

ASJC Scopus subject areas

  • Artificial Intelligence
  • Computer Vision and Pattern Recognition
  • Biomedical Engineering

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