A molecular marker diagnostic of a specific isolate of an arbuscular mycorrhizal fungus, Gigaspora margarita

Kazuhira Yokoyama, Takahiro Tateishi, Takuya Marumoto, Masanori Saito

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

To investigate the auto-ecology of a strain of Gigaspora margarita in a commercial inoculum, we found a pair of PCR primers amplifying a sequence of 235 bp diagnostic of the isolate. We designed an oligonucleotide probe based on the DNA sequence. The combination of PCR and the probing successfully detected the diagnostic sequence from both DNA preparations of single spores and colonized roots. This protocol enabled us to distinguish the isolate among several isolates from Japan, Nepal and the USA.

Original languageEnglish
Pages (from-to)171-175
Number of pages5
JournalFEMS Microbiology Letters
Volume212
Issue number2
DOIs
Publication statusPublished - 2002 Jul 2

Keywords

  • Diagnostic sequence
  • Gigaspora margarita
  • Isolate-specific identification
  • Oligonucleotide probe

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'A molecular marker diagnostic of a specific isolate of an arbuscular mycorrhizal fungus, Gigaspora margarita'. Together they form a unique fingerprint.

Cite this