A modified bait and switch strategy for the generation of esterolytic abzymes using concerted catalytic mechanisms

Yoshiharu Iwabuchi, Shinwa Kurihara, Motoko Oda, Ikuo Fujii

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Esterolytic abzymes were generated via immunization with a haptenic diphosphonate mounted with two distinct traps actuated by the 'bait and switch' and 'transition-state mimic' concepts. One monoclonal antibody, 12F12, was characterized in detail, and showed significant rate enhancement and unique substrate specificity. Experimental data support that the antibody catalyzes the reactions with a concerted catalytic mechanism.

Original languageEnglish
Pages (from-to)5341-5344
Number of pages4
JournalTetrahedron Letters
Volume40
Issue number29
DOIs
Publication statusPublished - 1999 Jul 16
Externally publishedYes

Keywords

  • Amino acids and derivatives
  • Antibodies
  • Catalysis
  • Ester hydrolysis

ASJC Scopus subject areas

  • Biochemistry
  • Drug Discovery
  • Organic Chemistry

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