A millisecond structured illumination microscope for super-resolution live cell imaging

Tomu Suzuki, Shinji Kajimoto, Narufumi Kitamura, Mayumi Takano-Kasuya, Naoko Furusawa, Yasushi Nakano, Hiroshi Fukumura, Kohsuke Gonda, Takakazu Nakabayashi

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

In typical super-resolution microscopy, high temporal resolution is sacrificed to achieve a spatial resolution beyond the diffraction limit. In this study, we have developed a new structured illumination microscopy (SIM) system using two Pockels cells, achieving a temporal resolution of 1 ms. The introduction of the two Pockels cells enables control of the phases of the illumination pattern along the X- A nd Y-axes in microseconds. We obtained five images consecutively with different illumination patterns and reconstructed a super-resolution image. We observed fluorescent nanoparticles in living cells for 8 s with super resolution and a frame rate of 1,000 fps.

Original languageEnglish
Article number045002
JournalApplied Physics Express
Volume13
Issue number4
DOIs
Publication statusPublished - 2020 Apr 1

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

Fingerprint Dive into the research topics of 'A millisecond structured illumination microscope for super-resolution live cell imaging'. Together they form a unique fingerprint.

Cite this