A microfluidic system for the study of the response of endothelial cells under pressure

Lei Li; Yang Yang; Xuetao Shi; Hongkai Wu; Hongda Chen; Jing Liu

doi:10.1007/s10404-013-1275-9

A microfluidic system for the study of the response of endothelial cells under pressure

Lei Li, Yang Yang, Xuetao Shi, Hongkai Wu, Hongda Chen, Jing Liu

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Hydrostatic pressure can affect the structure and function of endothelial cells (ECs). A microfluidic system was built to study how ECs respond to applied pressure. The system included a syringe pump, a PDMS-glass microfluidic chip, and a digital manometer for pressure monitoring. The manometer was connected with the chip in two ways (one was before the inlet and the other after the outlet of the microchannel). The static control and flowing control systems were also set up. Human umbilical vein endothelial cells (HUVECs) were cultured in the 4 cm × 2 mm × 100 μm channel. Pressure of 12 ± 0.5 or 18 ± 0.5 kPa was applied on the cells for 8 h. The F-actin cytoskeleton and the nuclei of the cells were stained for examination and endothelin-1 (ET-1) released from the cells in the channel was assayed by ELISA. The results showed that the cell area and ET-1 concentration increased with the pressure and a higher pressure caused more damages to the cells. This microfluidic system provides a convenient and cost-effective platform for the studies of cell response to pressure.

Original language	English
Pages (from-to)	1089-1096
Number of pages	8
Journal	Microfluidics and Nanofluidics
Volume	16
Issue number	6
DOIs	https://doi.org/10.1007/s10404-013-1275-9
Publication status	Published - 2014 Jun

Keywords

Endothelial cells
Microfluidic system
Pressure
Shear stress

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Materials Chemistry

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title = "A microfluidic system for the study of the response of endothelial cells under pressure",

abstract = "Hydrostatic pressure can affect the structure and function of endothelial cells (ECs). A microfluidic system was built to study how ECs respond to applied pressure. The system included a syringe pump, a PDMS-glass microfluidic chip, and a digital manometer for pressure monitoring. The manometer was connected with the chip in two ways (one was before the inlet and the other after the outlet of the microchannel). The static control and flowing control systems were also set up. Human umbilical vein endothelial cells (HUVECs) were cultured in the 4 cm × 2 mm × 100 μm channel. Pressure of 12 ± 0.5 or 18 ± 0.5 kPa was applied on the cells for 8 h. The F-actin cytoskeleton and the nuclei of the cells were stained for examination and endothelin-1 (ET-1) released from the cells in the channel was assayed by ELISA. The results showed that the cell area and ET-1 concentration increased with the pressure and a higher pressure caused more damages to the cells. This microfluidic system provides a convenient and cost-effective platform for the studies of cell response to pressure.",

keywords = "Endothelial cells, Microfluidic system, Pressure, Shear stress",

author = "Lei Li and Yang Yang and Xuetao Shi and Hongkai Wu and Hongda Chen and Jing Liu",

year = "2014",

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T1 - A microfluidic system for the study of the response of endothelial cells under pressure

AU - Li, Lei

AU - Yang, Yang

AU - Shi, Xuetao

AU - Wu, Hongkai

AU - Chen, Hongda

AU - Liu, Jing

PY - 2014/6

Y1 - 2014/6

N2 - Hydrostatic pressure can affect the structure and function of endothelial cells (ECs). A microfluidic system was built to study how ECs respond to applied pressure. The system included a syringe pump, a PDMS-glass microfluidic chip, and a digital manometer for pressure monitoring. The manometer was connected with the chip in two ways (one was before the inlet and the other after the outlet of the microchannel). The static control and flowing control systems were also set up. Human umbilical vein endothelial cells (HUVECs) were cultured in the 4 cm × 2 mm × 100 μm channel. Pressure of 12 ± 0.5 or 18 ± 0.5 kPa was applied on the cells for 8 h. The F-actin cytoskeleton and the nuclei of the cells were stained for examination and endothelin-1 (ET-1) released from the cells in the channel was assayed by ELISA. The results showed that the cell area and ET-1 concentration increased with the pressure and a higher pressure caused more damages to the cells. This microfluidic system provides a convenient and cost-effective platform for the studies of cell response to pressure.

AB - Hydrostatic pressure can affect the structure and function of endothelial cells (ECs). A microfluidic system was built to study how ECs respond to applied pressure. The system included a syringe pump, a PDMS-glass microfluidic chip, and a digital manometer for pressure monitoring. The manometer was connected with the chip in two ways (one was before the inlet and the other after the outlet of the microchannel). The static control and flowing control systems were also set up. Human umbilical vein endothelial cells (HUVECs) were cultured in the 4 cm × 2 mm × 100 μm channel. Pressure of 12 ± 0.5 or 18 ± 0.5 kPa was applied on the cells for 8 h. The F-actin cytoskeleton and the nuclei of the cells were stained for examination and endothelin-1 (ET-1) released from the cells in the channel was assayed by ELISA. The results showed that the cell area and ET-1 concentration increased with the pressure and a higher pressure caused more damages to the cells. This microfluidic system provides a convenient and cost-effective platform for the studies of cell response to pressure.

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KW - Microfluidic system

KW - Pressure

KW - Shear stress

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