A microfludic device with interdigitated array electrodes for detection of hormone active chemicals using genetically-engineered yeast cells

Kosuke Ino, Yusuke Kitagawa, Hitoshi Shiku, Masahiro Koide, Yoshiko Horiguchi, Tomoaki Itayama, Tomoyuki Yasukawa, Tomokazu Matsue

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Endocrine disrupting chemicals that act like hormones is a potential hazard for human health. Therefore, it is necessary to develop a device that can detect easily hormone active chemicals. In this study, a microfludic device with some analytical chambers was fabricated for manipulating the yeast cells that were genetically engineered to respond to the presence of hormone active chemicals by synthesizing β-galactosidase (β-gal). Interdigitated array (IDA) electrodes with 40 electrode fingers were incorporated into the device for sensitive detection of the β-gal activity electrochemically, and hormone active chemicals were successfully detected by using the systems.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1623-1625
Number of pages3
ISBN (Print)9780979806421
Publication statusPublished - 2009 Jan 1
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: 2009 Nov 12009 Nov 5

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Country/TerritoryKorea, Republic of
CityJeju
Period09/11/109/11/5

Keywords

  • Hormone active chemical
  • IDA electrode
  • Microfluidic device
  • Two-hybrid assay system
  • β-gal activity

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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