A microbial chip for bioassay was fabricated and its performance was characterized by scanning electrochemical microscopy (SECM). The microbial chip was fabricated by filling the micropores on a glass substrate with collagen gel embedding Escherichia coli (E.coli) cells. For measuring the activity of E.coli cells, the SECM measurement using ferricyanide as an electron mediator showed that the respiration activity of the immobilized E.coli cells increased with the concentration of glucose in the solution. The results suggested that the microbial chip can be used as a chemical sensor for nutritive species.
- Collagen Gel Microstructure
- Glucose Sensing
- Microbial Chip
- Scanning Electrochemical Microscopy
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