A microarray-based method for detecting methylated loci

I. Hatada, A. Kato, S. Morita, Y. Obata, K. Nagaoka, A. Sakurada, M. Sato, A. Horii, A. Tsujimoto, K. Matsubara

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

CpG island DNA methylation plays an important role in regulating gene expression in development and carcinogenesis. We developed a new microarray-based method called methylation amplification DNA chip (MAD) for detecting differences in methylation. In this method, only methylated CpG islands from the two samples that we wanted to compare were amplified and used for hybridization. The resource material for the microarray was derived from the methylated DNA library of the sample in which we wanted to detect hypermethylation. Choosing the methylated DNA library as the resource material of the microarray increased the percentage of DNA fragments derived from hypermethylated loci on the microarray.

Original languageEnglish
Pages (from-to)448-451
Number of pages4
JournalJournal of Human Genetics
Volume47
Issue number8
DOIs
Publication statusPublished - 2002
Externally publishedYes

Keywords

  • CpG island
  • DNA chip
  • DNA methylation
  • Hypermethylation
  • Microarray

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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