A micro-channel-well system for culture and differentiation of embryonic stem cells on different types of substrate

Li Liu, Chunxiong Luo, Xiaofeng Ni, Li Wang, Kaori Yamauchi, Shinichi M. Nomura, Norio Nakatsuji, Yong Chen

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

We have developed a combined micro-channel and micro-well system for easy cell loading, culture and post-culture operation on a chip. To demonstrate the reliability of the system, on chip cell culture and differentiation were performed with different types of substrates made of culture dish, glass cover slide and polydimethylsiloaxe (PDMS). As expected, mouse embryo fibroblasts (MEF) showed different adhesion and growth rate on different substrates. When embryonic stem (ES) cells were co-cultured with MEFs, the formation of ES colonies is efficient on both glass and Petri dish, although PDMS could also be used. Finally, ES cell differentiation with neuron growth factors was performed on different substrates, showing clear advantages of using culture Petri dish over both glass and PDMS.

Original languageEnglish
Pages (from-to)505-511
Number of pages7
JournalBiomedical microdevices
Volume12
Issue number3
DOIs
Publication statusPublished - 2010 Jun
Externally publishedYes

Keywords

  • Cell culture
  • Cell differentiation
  • Embryonic stem cells
  • Microfluidics
  • Substrate

ASJC Scopus subject areas

  • Biomedical Engineering
  • Molecular Biology

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