A method for separation and determination of cytokinin nucleotides from plant tissues

Kentaro Takei, Tomoyuki Yamaya, Hitoshi Sakakibara

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Recent progress in the study of cytokinin metabolism in plants indicates that quantitative analysis of cytokinin nucleotides is essential for elucidation of early steps of the biosynthetic pathway. However, traditional procedures for purification and quantification of cytokinin cannot discriminate the various nucleotides. We describe here a method for separation and determination of cytokinin nucleotides through a series of anion-exchange column chromatography steps followed by liquid chromatography-mass spectrometry. This method enabled us to analyze the amount of each species of cytokinin nucleotide in plant tissues.

Original languageEnglish
Pages (from-to)265-269
Number of pages5
JournalJournal of Plant Research
Volume116
Issue number3
DOIs
Publication statusPublished - 2003 Jun
Externally publishedYes

Keywords

  • Cytokinin nucleotides
  • Cytokinins
  • Isopentenyladenine
  • Isopentenyltransferase
  • Liquid chromatography-mass spectrometry
  • trans-Zeatin

ASJC Scopus subject areas

  • Plant Science

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