A method for determination of UDP-GlcNAc: GlcNAcβ1-6(GlcNAcβ1-2) Manα1-R [GlcNAc to Man] β1-4N-Acetylglucosaminyltransferase VI activity

Tomohiko Taguchi, Naoyuki Taniguchi

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

To characterize and purify glycosyltransferases, it is essential to establish a simple and sensitive assay method. Here, we describe a method for determination of the activity of GnT VI (UDP-GlcNAc: GlcNAcβ1- 6(GlcNAcβ1-2)Manα1-R [GlcNAc to Man] β1-4N- acetylglucosaminyltransferase VI) using a fluorescently labeled oligosaccharide.

Original languageEnglish
Title of host publicationGlycosyltransferases
Subtitle of host publicationMethods and Protocols
Pages299-305
Number of pages7
DOIs
Publication statusPublished - 2013 Jul 25
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1022
ISSN (Print)1064-3745

Keywords

  • Fluorescent labeling
  • HPLC
  • N-Acetylglucosaminyltransferase
  • Pentaantennary N-linked glycan
  • Pyridylamination

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Taguchi, T., & Taniguchi, N. (2013). A method for determination of UDP-GlcNAc: GlcNAcβ1-6(GlcNAcβ1-2) Manα1-R [GlcNAc to Man] β1-4N-Acetylglucosaminyltransferase VI activity. In Glycosyltransferases: Methods and Protocols (pp. 299-305). (Methods in Molecular Biology; Vol. 1022). https://doi.org/10.1007/978-1-62703-465-4-22