A Membrane-Anchored Protein Kinase Involved in Brassica Self-Incompatibility Signaling

Kohji Murase, Hiroshi Shiba, Megumi Iwano, Fang Sik Che, Masao Watanabe, Akira Isogai, Seiji Takayama

Research output: Contribution to journalArticle

173 Citations (Scopus)

Abstract

Self-incompatibility (SI) response in Brassica is initiated by haplotype-specific interactions between the pollen-borne ligand S locus protein 11/SCR and its stigmatic S receptor kinase, SRK. This binding induces autophosphorylation of SRK, which is then thought to trigger a signaling cascade that leads to self-pollen rejection. A recessive mutation of the modifier (m) gene eliminates the SI response in stigma. Positional cloning of M has revealed that it encodes a membrane-anchored cytoplasmic serine/threonine protein kinase, designated M locus protein kinase (MLPK). Transient expression of MLPK restores the ability of mm papilla cells to reject self-pollen, suggesting that MLPK is a positive mediator of Brassica SI signaling.

Original languageEnglish
Pages (from-to)1516-1519
Number of pages4
JournalScience
Volume303
Issue number5663
DOIs
Publication statusPublished - 2004 Mar 5
Externally publishedYes

ASJC Scopus subject areas

  • General

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    Murase, K., Shiba, H., Iwano, M., Che, F. S., Watanabe, M., Isogai, A., & Takayama, S. (2004). A Membrane-Anchored Protein Kinase Involved in Brassica Self-Incompatibility Signaling. Science, 303(5663), 1516-1519. https://doi.org/10.1126/science.1093586