A low-cost method for brain slice cultures

Ryuta Koyama; Rieko Muramatsu; Takuya Sasaki; Rie Kimura; Chihiro Ueyama; Makoto Tamura; Naohiro Tamura; Junya Ichikawa; Naoya Takahashi; Atsushi Usami; Maki K. Yamada; Norio Matsuki; Yuji Ikegaya

doi:10.1254/jphs.SC0070119

A low-cost method for brain slice cultures

Ryuta Koyama, Rieko Muramatsu, Takuya Sasaki, Rie Kimura, Chihiro Ueyama, Makoto Tamura, Naohiro Tamura, Junya Ichikawa, Naoya Takahashi, Atsushi Usami, Maki K. Yamada, Norio Matsuki, Yuji Ikegaya

Research output: Contribution to journal › Article › peer-review

56 Citations (Scopus)

Abstract

Low-cost, simple procedures for organotypic tissue cultures are desirable for high-throughput biological experiments such as large-scale medical/drug screening. We present a practical and economical method to cultivate brain slices using hydrophilic filtration membranes. With a cost reduction of more than 90%, this technique allows us to prepare hippocampal slice cultures that are morphologically and functionally indistinguishable from those obtained by the widely used Millicell-CM® method.

Original language	English
Pages (from-to)	191-194
Number of pages	4
Journal	Journal of Pharmacological Sciences
Volume	104
Issue number	2
DOIs	https://doi.org/10.1254/jphs.SC0070119
Publication status	Published - 2007
Externally published	Yes

Keywords

Hippocampal slice
Membrane-interface tissue culture
Organotypic culture

ASJC Scopus subject areas

Molecular Medicine
Pharmacology

Access to Document

10.1254/jphs.SC0070119

Cite this

@article{e3531b10cb5247129b0503e9f3027342,

title = "A low-cost method for brain slice cultures",

abstract = "Low-cost, simple procedures for organotypic tissue cultures are desirable for high-throughput biological experiments such as large-scale medical/drug screening. We present a practical and economical method to cultivate brain slices using hydrophilic filtration membranes. With a cost reduction of more than 90%, this technique allows us to prepare hippocampal slice cultures that are morphologically and functionally indistinguishable from those obtained by the widely used Millicell-CM{\textregistered} method.",

keywords = "Hippocampal slice, Membrane-interface tissue culture, Organotypic culture",

author = "Ryuta Koyama and Rieko Muramatsu and Takuya Sasaki and Rie Kimura and Chihiro Ueyama and Makoto Tamura and Naohiro Tamura and Junya Ichikawa and Naoya Takahashi and Atsushi Usami and Yamada, {Maki K.} and Norio Matsuki and Yuji Ikegaya",

year = "2007",

doi = "10.1254/jphs.SC0070119",

language = "English",

volume = "104",

pages = "191--194",

journal = "Journal of Pharmacological Sciences",

issn = "1347-8648",

publisher = "Japanese Pharmacological Society",

number = "2",

}

TY - JOUR

T1 - A low-cost method for brain slice cultures

AU - Koyama, Ryuta

AU - Muramatsu, Rieko

AU - Sasaki, Takuya

AU - Kimura, Rie

AU - Ueyama, Chihiro

AU - Tamura, Makoto

AU - Tamura, Naohiro

AU - Ichikawa, Junya

AU - Takahashi, Naoya

AU - Usami, Atsushi

AU - Yamada, Maki K.

AU - Matsuki, Norio

AU - Ikegaya, Yuji

PY - 2007

Y1 - 2007

N2 - Low-cost, simple procedures for organotypic tissue cultures are desirable for high-throughput biological experiments such as large-scale medical/drug screening. We present a practical and economical method to cultivate brain slices using hydrophilic filtration membranes. With a cost reduction of more than 90%, this technique allows us to prepare hippocampal slice cultures that are morphologically and functionally indistinguishable from those obtained by the widely used Millicell-CM® method.

AB - Low-cost, simple procedures for organotypic tissue cultures are desirable for high-throughput biological experiments such as large-scale medical/drug screening. We present a practical and economical method to cultivate brain slices using hydrophilic filtration membranes. With a cost reduction of more than 90%, this technique allows us to prepare hippocampal slice cultures that are morphologically and functionally indistinguishable from those obtained by the widely used Millicell-CM® method.

KW - Hippocampal slice

KW - Membrane-interface tissue culture

KW - Organotypic culture

UR - http://www.scopus.com/inward/record.url?scp=34250880712&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34250880712&partnerID=8YFLogxK

U2 - 10.1254/jphs.SC0070119

DO - 10.1254/jphs.SC0070119

M3 - Article

C2 - 17558179

AN - SCOPUS:34250880712

SN - 1347-8648

VL - 104

SP - 191

EP - 194

JO - Journal of Pharmacological Sciences

JF - Journal of Pharmacological Sciences

IS - 2

ER -

A low-cost method for brain slice cultures

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this