A low-cost method for brain slice cultures

Ryuta Koyama, Rieko Muramatsu, Takuya Sasaki, Rie Kimura, Chihiro Ueyama, Makoto Tamura, Naohiro Tamura, Junya Ichikawa, Naoya Takahashi, Atsushi Usami, Maki K. Yamada, Norio Matsuki, Yuji Ikegaya

Research output: Contribution to journalArticlepeer-review

56 Citations (Scopus)


Low-cost, simple procedures for organotypic tissue cultures are desirable for high-throughput biological experiments such as large-scale medical/drug screening. We present a practical and economical method to cultivate brain slices using hydrophilic filtration membranes. With a cost reduction of more than 90%, this technique allows us to prepare hippocampal slice cultures that are morphologically and functionally indistinguishable from those obtained by the widely used Millicell-CM® method.

Original languageEnglish
Pages (from-to)191-194
Number of pages4
JournalJournal of Pharmacological Sciences
Issue number2
Publication statusPublished - 2007
Externally publishedYes


  • Hippocampal slice
  • Membrane-interface tissue culture
  • Organotypic culture

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology


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