A long natural-antisense RNA is accumulated in the conidia of Aspergillus oryzae

Masaru Tsujii, Satoshi Okuda, Kazutomo Ishi, Kana Madokoro, Michio Takeuchi, Youhei Yamagata

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Analysis of expressed sequence tag libraries from various culture conditions revealed the existence of conidia-specific transcripts assembled to putative conidiation-specific reductase gene (csrA) in Aspergillus oryzae. However, the all transcripts were transcribed with opposite direction to the gene csrA. The sequence analysis of the transcript revealed that the RNA overlapped mRNA of csrA with 3'-end, and did not code protein longer than 60 amino acid residues. We designated the transcript Conidia Specific Long Natural-antisense RNA (CSLNR). The real-time PCR analysis demonstrated that the CSLNR is conidia-specific transcript, which cannot be transcribed in the absence of brlA, and the amount of CSLNR was much more than that of the transcript from csrA in conidia. Furthermore, the csrA deletion, also lacking coding region of CSLNR in A. oryzae reduced the number of conidia. Overexpression of CsrA demonstrated the inhibition of growth and conidiation, while CSLNR did not affect conidiation.

Original languageEnglish
Pages (from-to)386-398
Number of pages13
JournalBioscience, Biotechnology and Biochemistry
Volume80
Issue number2
DOIs
Publication statusPublished - 2016
Externally publishedYes

Keywords

  • Aspergillus oryzae
  • CSLNR
  • Long natural antisense RNA

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Fingerprint

Dive into the research topics of 'A long natural-antisense RNA is accumulated in the conidia of Aspergillus oryzae'. Together they form a unique fingerprint.

Cite this