A linkage map for sugi (Cryptomeria japonica) based on RFLP, RAPD, and isozyme loci

Y. Mukai, Y. Suyama, Y. Tsumura, T. Kawahara, H. Yoshimaru, T. Kondo, N. Tomaru, N. Kuramoto, M. Murai

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

A linkage map for sugi was constructed on the basis of restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), and isozyme loci using a three-generation pedigree prepared for genetic analysis of heartwood color. A total of 128 RFLP (123 cDNA and 5 genomic probes), 33 RAPD, 2 isozyme, and 1 morphological (dwarf) loci segregated in 73 progeny. Of the 164 segregating loci, 145 loci were distributed in 20 linkage groups. Of these loci, 91 with confirmed map positions were assigned to 13 linkage groups, covering a total of 887.3 cM. A clustering of markers with distorted segregation was observed in 6 linkage groups. In the four clusters, distortions with a reduction in the number of homozygotes from one parent only were found.

Original languageEnglish
Pages (from-to)835-840
Number of pages6
JournalTheoretical and Applied Genetics
Volume90
Issue number6
DOIs
Publication statusPublished - 1995 May 1
Externally publishedYes

Keywords

  • Linkage map
  • RAPD
  • RFLP
  • Sugi (Cryptomeria japonica)
  • Three-generation pedigree

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

Fingerprint Dive into the research topics of 'A linkage map for sugi (Cryptomeria japonica) based on RFLP, RAPD, and isozyme loci'. Together they form a unique fingerprint.

  • Cite this

    Mukai, Y., Suyama, Y., Tsumura, Y., Kawahara, T., Yoshimaru, H., Kondo, T., Tomaru, N., Kuramoto, N., & Murai, M. (1995). A linkage map for sugi (Cryptomeria japonica) based on RFLP, RAPD, and isozyme loci. Theoretical and Applied Genetics, 90(6), 835-840. https://doi.org/10.1007/BF00222019