A lectin array analysis for wild-type and α-Gal-knockout pig islets versus healthy human islets

Shuji Miyagawa, Akira Maeda, Shunsaku Takeishi, Takehisa Ueno, Noriaki Usui, Shinichi Matsumoto, Teru Okitsu, Masafumi Goto, Hiroshi Nagashima

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Purpose: We performed lectin microarray analyses of islets from wild-type (WT) pigs and a1-3galactosyltransferase gene knockout (GKO) pigs and compared the results with the corresponding values for islets from healthy humans. Methods: Islets were isolated from the pancreas. After sonication and centrifugation, the proteins in the supernatant from each islet were labeled with Cy3 and applied to a lectin array. Results: Despite negligible expression of the Gal antigen on the adult pig islets (APIs), GKO-islets showed weaker signals, not only for GS-I-B4 but also for PNA, WFA, PTL-I, and GS-I-A4, than the WT islets, indicating reduced contents of α-linked GalNAc and Galβ1-3GalNAc. In comparing the islets of pigs vs. humans, human islets showed stronger signals for UEA-I, AAL, TJA-II, EEL, WFA, HPA, DBA, SBA and PTL-I, indicating that besides ABO blood type antigens, high levels of fucose and α-linked GalNAc are present. On the other hand, the high mannose form was very rich in the APIs. Conclusion: GKO reduced alpha-linked GalNAc, despite negligible expression of the Gal antigen on WT-API. On the other hand, the high-mannose form was richer in both APIs than in healthy human islets. These results provide useful information for future studies.

Original languageEnglish
Pages (from-to)1439-1447
Number of pages9
JournalSurgery today
Volume43
Issue number12
DOIs
Publication statusPublished - 2013 Apr 3

Keywords

  • Glycoantigen
  • Islets
  • Lectin array
  • Xenotransplantation

ASJC Scopus subject areas

  • Surgery

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    Miyagawa, S., Maeda, A., Takeishi, S., Ueno, T., Usui, N., Matsumoto, S., Okitsu, T., Goto, M., & Nagashima, H. (2013). A lectin array analysis for wild-type and α-Gal-knockout pig islets versus healthy human islets. Surgery today, 43(12), 1439-1447. https://doi.org/10.1007/s00595-013-0569-6