A large, square-shaped, DNA origami nanopore with sealing function on a giant vesicle membrane

Shoji Iwabuchi; Ibuki Kawamata; Satoshi Murata; Shin Ichiro M. Nomura

doi:10.1039/d0cc07412h

A large, square-shaped, DNA origami nanopore with sealing function on a giant vesicle membrane

Shoji Iwabuchi, Ibuki Kawamata, Satoshi Murata, Shin Ichiro M. Nomura

ENG - Robotics

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Intaking molecular information from the external environment is essential for the normal functioning of artificial cells/molecular robots. Herein, we report the design and function of a membrane nanopore using a DNA origami square tube with a cross-section of 100 nm². When the nanopore is added to a giant vesicle that mimics a cell membrane, the permeation of large external hydrophilic fluorescent molecules is observed. Furthermore, the addition of up to four ssDNA strands enables size-based selective transport of molecules. A controllable artificial nanopore should facilitate the communication between the vesicle components and their environment.

Original language	English
Pages (from-to)	2990-2993
Number of pages	4
Journal	Chemical Communications
Volume	57
Issue number	24
DOIs	https://doi.org/10.1039/d0cc07412h
Publication status	Published - 2021 Mar 25

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

Access to Document

10.1039/d0cc07412h

Cite this

@article{4097bc4957e040fb83db38e0032fdc6a,

title = "A large, square-shaped, DNA origami nanopore with sealing function on a giant vesicle membrane",

abstract = "Intaking molecular information from the external environment is essential for the normal functioning of artificial cells/molecular robots. Herein, we report the design and function of a membrane nanopore using a DNA origami square tube with a cross-section of 100 nm2. When the nanopore is added to a giant vesicle that mimics a cell membrane, the permeation of large external hydrophilic fluorescent molecules is observed. Furthermore, the addition of up to four ssDNA strands enables size-based selective transport of molecules. A controllable artificial nanopore should facilitate the communication between the vesicle components and their environment.",

author = "Shoji Iwabuchi and Ibuki Kawamata and Satoshi Murata and Nomura, {Shin Ichiro M.}",

note = "Funding Information: This work was supported by the JSPS and MEXT KAKENHI (grant numbers JP20H05701, JP20H00619, 20H00618, 18K18144, 19KK0261, and 17H00769); the FRIS Program for Creative Interdisciplinary Research; and AMED-CREST 20gm0810001h0106. Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2021.",

year = "2021",

month = mar,

day = "25",

doi = "10.1039/d0cc07412h",

language = "English",

volume = "57",

pages = "2990--2993",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "24",

}

TY - JOUR

T1 - A large, square-shaped, DNA origami nanopore with sealing function on a giant vesicle membrane

AU - Iwabuchi, Shoji

AU - Kawamata, Ibuki

AU - Murata, Satoshi

AU - Nomura, Shin Ichiro M.

N1 - Funding Information: This work was supported by the JSPS and MEXT KAKENHI (grant numbers JP20H05701, JP20H00619, 20H00618, 18K18144, 19KK0261, and 17H00769); the FRIS Program for Creative Interdisciplinary Research; and AMED-CREST 20gm0810001h0106. Publisher Copyright: © The Royal Society of Chemistry 2021.

PY - 2021/3/25

Y1 - 2021/3/25

N2 - Intaking molecular information from the external environment is essential for the normal functioning of artificial cells/molecular robots. Herein, we report the design and function of a membrane nanopore using a DNA origami square tube with a cross-section of 100 nm2. When the nanopore is added to a giant vesicle that mimics a cell membrane, the permeation of large external hydrophilic fluorescent molecules is observed. Furthermore, the addition of up to four ssDNA strands enables size-based selective transport of molecules. A controllable artificial nanopore should facilitate the communication between the vesicle components and their environment.

AB - Intaking molecular information from the external environment is essential for the normal functioning of artificial cells/molecular robots. Herein, we report the design and function of a membrane nanopore using a DNA origami square tube with a cross-section of 100 nm2. When the nanopore is added to a giant vesicle that mimics a cell membrane, the permeation of large external hydrophilic fluorescent molecules is observed. Furthermore, the addition of up to four ssDNA strands enables size-based selective transport of molecules. A controllable artificial nanopore should facilitate the communication between the vesicle components and their environment.

UR - http://www.scopus.com/inward/record.url?scp=85103057627&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85103057627&partnerID=8YFLogxK

U2 - 10.1039/d0cc07412h

DO - 10.1039/d0cc07412h

M3 - Article

C2 - 33587063

AN - SCOPUS:85103057627

SN - 1359-7345

VL - 57

SP - 2990

EP - 2993

JO - Chemical Communications

JF - Chemical Communications

IS - 24

ER -

A large, square-shaped, DNA origami nanopore with sealing function on a giant vesicle membrane

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this