A label-free and sensitive fluorescent method for the detection of uracil-DNA glycosylase activity

Jing Tao; Panshu Song; Yusuke Sato; Seiichi Nishizawa; Norio Teramae; Aijun Tong; Yu Xiang

doi:10.1039/c4cc06170e

A label-free and sensitive fluorescent method for the detection of uracil-DNA glycosylase activity

Jing Tao, Panshu Song, Yusuke Sato, Seiichi Nishizawa, Norio Teramae, Aijun Tong, Yu Xiang

SCI - Chemistry

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

The activity of uracil-DNA glycosylase (UDG), an enzyme in the base excision repair, is detected at a high sensitivity by a DNA substrate containing only one uracil through a label-free fluorescent approach, which is also successfully applied for the measurement of UDG inhibitors.

Original language	English
Pages (from-to)	929-932
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	5
DOIs	https://doi.org/10.1039/c4cc06170e
Publication status	Published - 2015 Jan 18

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/c4cc06170e

Cite this

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title = "A label-free and sensitive fluorescent method for the detection of uracil-DNA glycosylase activity",

abstract = "The activity of uracil-DNA glycosylase (UDG), an enzyme in the base excision repair, is detected at a high sensitivity by a DNA substrate containing only one uracil through a label-free fluorescent approach, which is also successfully applied for the measurement of UDG inhibitors.",

author = "Jing Tao and Panshu Song and Yusuke Sato and Seiichi Nishizawa and Norio Teramae and Aijun Tong and Yu Xiang",

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AU - Tao, Jing

AU - Song, Panshu

AU - Sato, Yusuke

AU - Nishizawa, Seiichi

AU - Teramae, Norio

AU - Tong, Aijun

AU - Xiang, Yu

PY - 2015/1/18

Y1 - 2015/1/18

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A label-free and sensitive fluorescent method for the detection of uracil-DNA glycosylase activity

Abstract

ASJC Scopus subject areas

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