A key inactivation factor of HeLa cell viability by a plasma flow

Takehiko Sato, Mayo Yokoyama, Kohei Johkura

Research output: Contribution to journalArticlepeer-review

68 Citations (Scopus)

Abstract

Recently, a plasma flow has been applied to medical treatment using effects of various kinds of stimuli such as chemical species, charged particles, heat, light, shock wave and electric fields. Among them, the chemical species are known to cause an inactivation of cell viability. However, the mechanisms and key factors of this event are not yet clear. In this study, we focused on the effect of H2O2 in plasma-treated culture medium because it is generated in the culture medium and it is also chemically stable compared with free radicals generated by the plasma flow. To elucidate the significance of H2O2, we assessed the differences in the effects of plasma-treated medium and H2O2-added medium against inactivation of HeLa cell viability. These two media showed comparable effects on HeLa cells in terms of the survival ratios, morphological features of damage processes, permeations of H2O2 into the cells, response to H2O2 decomposition by catalase and comprehensive gene expression. The results supported that among chemical species generated in a plasma-treated culture medium, H2O2 is one of the main factors responsible for inactivation of HeLa cell viability.

Original languageEnglish
Article number372001
JournalJournal of Physics D: Applied Physics
Volume44
Issue number37
DOIs
Publication statusPublished - 2011 Sep 21

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Acoustics and Ultrasonics
  • Surfaces, Coatings and Films

Fingerprint

Dive into the research topics of 'A key inactivation factor of HeLa cell viability by a plasma flow'. Together they form a unique fingerprint.

Cite this