A hydroxysteroid sulfotransferase, St2b2, is a skin cholesterol sulfotransferase in mice

Miki Shimada, Yoshiteru Kamiyama, Akira Sato, Wataru Honma, Kiyoshi Nagata, Yasushi Yamazoe

    Research output: Contribution to journalArticlepeer-review

    11 Citations (Scopus)

    Abstract

    The mRNA of a sulfotransferase (St2b2) mediating cholesterol sulfation was detected in mouse skin. Recombinant St2b2 also mediated the sulfation of pregnenolone, 3β-hydroxy-5-cholen-24-oic acid, and dehydroepiandrosterone. St2b2 protein was detected in skin cytosols on Western blotting. The addition of 10 nM TPA to skin epidermal cells from newborn mice resulted in a twofold increase in cholesterol sulfation and concomitantly enhanced the St2b2 content after 40 h. Other candidate cholesterol sulfotransferases, St2a4 and St2a9, were not detected in skin by RT-PCR. These results indicate that St2b2 is a cholesterol sulfotransferase in mouse skin.

    Original languageEnglish
    Pages (from-to)167-169
    Number of pages3
    JournalJournal of biochemistry
    Volume131
    Issue number2
    DOIs
    Publication statusPublished - 2002 Jan 1

    Keywords

    • Cholesterol sulfation
    • Epidermal cell
    • Hydroxysteroid sulfotransferase
    • Mouse skin
    • Phorbol ester

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology

    Fingerprint Dive into the research topics of 'A hydroxysteroid sulfotransferase, St2b2, is a skin cholesterol sulfotransferase in mice'. Together they form a unique fingerprint.

    Cite this