A hydroxysteroid sulfotransferase, St2b2, is a skin cholesterol sulfotransferase in mice

Miki Shimada, Yoshiteru Kamiyama, Akira Sato, Wataru Honma, Kiyoshi Nagata, Yasushi Yamazoe

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

The mRNA of a sulfotransferase (St2b2) mediating cholesterol sulfation was detected in mouse skin. Recombinant St2b2 also mediated the sulfation of pregnenolone, 3β-hydroxy-5-cholen-24-oic acid, and dehydroepiandrosterone. St2b2 protein was detected in skin cytosols on Western blotting. The addition of 10 nM TPA to skin epidermal cells from newborn mice resulted in a twofold increase in cholesterol sulfation and concomitantly enhanced the St2b2 content after 40 h. Other candidate cholesterol sulfotransferases, St2a4 and St2a9, were not detected in skin by RT-PCR. These results indicate that St2b2 is a cholesterol sulfotransferase in mouse skin.

Original languageEnglish
Pages (from-to)167-169
Number of pages3
JournalJournal of biochemistry
Volume131
Issue number2
DOIs
Publication statusPublished - 2002
Externally publishedYes

Keywords

  • Cholesterol sulfation
  • Epidermal cell
  • Hydroxysteroid sulfotransferase
  • Mouse skin
  • Phorbol ester

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint

Dive into the research topics of 'A hydroxysteroid sulfotransferase, St2b2, is a skin cholesterol sulfotransferase in mice'. Together they form a unique fingerprint.

Cite this