A histone chaperone, DEK, transcriptionally coactivates a nuclear receptor

Shun Sawatsubashi, Takuya Murata, Jinseon Lim, Ryoji Fujiki, Saya Ito, Eriko Suzuki, Masahiko Tanabe, Yue Zhao, Shuhei Kimura, Sally Fujiyama, Takashi Ueda, Daiki Umetsu, Takashi Ito, Ken Ichi Takeyama, Shigeaki Kato

Research output: Contribution to journalArticlepeer-review

113 Citations (Scopus)


Chromatin reorganization is essential for transcriptional control by sequence-specific transcription factors. However, the molecular link between transcriptional control and chromatin reconfiguration remains unclear. By colocalization of the nuclear ecdysone receptor (EcR) on the ecdysone-induced puff in the salivary gland, Drosophila DEK (dDEK) was genetically identified as a coactivator of EcR in both insect cells and intact flies. Biochemical purification and characterization of the complexes containing fly and human DEKs revealed that DEKs serve as histone chaperones via phosphorylation by forming complexes with casein kinase 2. Consistent with the preferential association of the DEK complex with histones enriched in active epigenetic marks, dDEK facilitated H3.3 assembly during puff formation. In some human myeloid leukemia patients, DEK was fused to CAN by chromosomal translocation. This mutation significantly reduced formation of the DEK complex, which is required for histone chaperone activity. Thus, the present study suggests that at least one histone chaperone can be categorized as a type of transcriptional coactivator for nuclear receptors.

Original languageEnglish
Pages (from-to)159-170
Number of pages12
JournalGenes and Development
Issue number2
Publication statusPublished - 2010 Jan 15
Externally publishedYes


  • Acute myeloid leukemia
  • Coactivator
  • DEK
  • Ecdysone receptor
  • Histone chaperone
  • Histone variant H3.3

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology


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