TY - JOUR
T1 - A highly sensitive method for determining both Mn- and CuZn superoxide dismutase activities in tissues and blood cells
AU - Nakano, Minoru
AU - Kimura, Hirokazu
AU - Hara, Masanori
AU - Kuroiwa, Minoru
AU - Kato, Masahiko
AU - Totsune, Kazuhito
AU - Yoshikawa, Toshikazu
PY - 1990/6
Y1 - 1990/6
N2 - A highly sensitive method for determining the superoxide dismutase (EC 1.15.1.1) in various tissues and blood cells is described. This method involves inhibition of a cypridina luciferin analog that is chemiluminescence dependent upon O2- generated by hypoxanthinexanthine oxidase. Manganeous superoxide dismutase, which is sensitive to sodium dodecyl sulfate, was determined and calculated by subtraction of superoxide dismutase activity in tissue extract treated with this detergent (CuZn superoxide dismutase) from that in untreated tissue extract (total superoxide dismutase). Both Mn- and CuZn superoxide dismutase activities were expressed as equivalent nanograms of bovine erythrocyte superoxide dismutase per milliliter. Sensitivity limits of the chemiluminescence methods with 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one and 2-methyl-6-phenyl-3,7-dihydroimidazo[1,2-a]pyrazin-3-one as cypridina luciferin analogs were 1 ng and 2-3 ng of superoxide dismutase/ml, respectively.
AB - A highly sensitive method for determining the superoxide dismutase (EC 1.15.1.1) in various tissues and blood cells is described. This method involves inhibition of a cypridina luciferin analog that is chemiluminescence dependent upon O2- generated by hypoxanthinexanthine oxidase. Manganeous superoxide dismutase, which is sensitive to sodium dodecyl sulfate, was determined and calculated by subtraction of superoxide dismutase activity in tissue extract treated with this detergent (CuZn superoxide dismutase) from that in untreated tissue extract (total superoxide dismutase). Both Mn- and CuZn superoxide dismutase activities were expressed as equivalent nanograms of bovine erythrocyte superoxide dismutase per milliliter. Sensitivity limits of the chemiluminescence methods with 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin-3-one and 2-methyl-6-phenyl-3,7-dihydroimidazo[1,2-a]pyrazin-3-one as cypridina luciferin analogs were 1 ng and 2-3 ng of superoxide dismutase/ml, respectively.
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U2 - 10.1016/0003-2697(90)90457-K
DO - 10.1016/0003-2697(90)90457-K
M3 - Article
C2 - 2200307
AN - SCOPUS:0025299216
VL - 187
SP - 277
EP - 280
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 2
ER -