TY - JOUR
T1 - A highly-sensitive l-lactate biosensor based on sol-gel film combined with multi-walled carbon nanotubes (MWCNTs) modified electrode
AU - Huang, Jiadong
AU - Song, Zhao
AU - Li, Jing
AU - Yang, Yu
AU - Shi, Haibin
AU - Wu, Baoyan
AU - Anzai, Jun ichi
AU - Osa, Tetsuo
AU - Chen, Qiang
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of People's Republic of China (No. 90209054), the Project 863 (No. 2002AA326060) and the Grant-in-Aid for the High-Tech Cooperation Project of Nankai University and Tianjin University from the Chinese Ministry of Education.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2007/1
Y1 - 2007/1
N2 - A new type of amperometric l-lactate biosensor based on silica sol-gel and multi-walled carbon nanotubes (MWCNTs) organic-inorganic hybrid composite material was developed. The sol-gel film was used to immobilize l-lactate oxidase on the surface of glassy carbon electrode (GCE). MWCNTs were used to increase the current response and improve the performance of biosensor. The sol-gel film fabrication process parameters such as H2O : TEOS and pH were optimized, Effects of some experimental variables such as applied potential, temperature, and pH on the current response of the biosensor were investigated. Analytical characteristics and dynamic parameters of the biosensors with and without MWCNTs in the hybrid film were compared, and the results showed that analytical performance of the biosensor could be improved greatly after introduction of the MWCNTs. Sensitivity, linear range, limit of detection (S / N = 3) were 2.097 μA mM- 1, 0.3 to 1.5 mM, 0.8 × 10- 3 mM for the biosensor without MWCNTs and 6.031 μA mM- 1, 0.2 to 2.0 mM, 0.3 × 10- 3 mM for the biosensor with MWCNTs, respectively. This method has been used to determine the l-lactate concentration in real human blood samples.
AB - A new type of amperometric l-lactate biosensor based on silica sol-gel and multi-walled carbon nanotubes (MWCNTs) organic-inorganic hybrid composite material was developed. The sol-gel film was used to immobilize l-lactate oxidase on the surface of glassy carbon electrode (GCE). MWCNTs were used to increase the current response and improve the performance of biosensor. The sol-gel film fabrication process parameters such as H2O : TEOS and pH were optimized, Effects of some experimental variables such as applied potential, temperature, and pH on the current response of the biosensor were investigated. Analytical characteristics and dynamic parameters of the biosensors with and without MWCNTs in the hybrid film were compared, and the results showed that analytical performance of the biosensor could be improved greatly after introduction of the MWCNTs. Sensitivity, linear range, limit of detection (S / N = 3) were 2.097 μA mM- 1, 0.3 to 1.5 mM, 0.8 × 10- 3 mM for the biosensor without MWCNTs and 6.031 μA mM- 1, 0.2 to 2.0 mM, 0.3 × 10- 3 mM for the biosensor with MWCNTs, respectively. This method has been used to determine the l-lactate concentration in real human blood samples.
KW - Amperometric biosensor
KW - Chemically modified electrode
KW - Multi-walled carbon nanotubes
KW - Sol-gel
KW - l-lactate oxidase
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U2 - 10.1016/j.msec.2006.01.001
DO - 10.1016/j.msec.2006.01.001
M3 - Article
AN - SCOPUS:33751511783
VL - 27
SP - 29
EP - 34
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
SN - 0928-4931
IS - 1
ER -