A Helical String of Alternately Connected Three-Helix Bundles for the Cell Wall-Associated Adhesion Protein Ebh from Staphylococcus aureus

Yoshikazu Tanaka, Sou Sakamoto, Makoto Kuroda, Shuichiro Goda, Yong Gui Gao, Kouhei Tsumoto, Yuzuru Hiragi, Min Yao, Nobuhisa Watanabe, Toshiko Ohta, Isao Tanaka

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

The 1.1 MDa cell-wall-associated adhesion protein of staphylococci, Ebh, consists of several distinct regions, including a large central region with 52 imperfect repeats of 126 amino acid residues. We investigated the structure of this giant molecule by X-ray crystallography, circular dichroism (CD) spectrometry, and small-angle X-ray scattering (SAXS). The crystal structure of two repeats showed that each repeat consists of two distinct three-helix bundles, and two such repeats are connected along the long axis, resulting in a rod-like structure that is 120 Å in length. CD and SAXS analyses of the samples with longer repeats suggested that each repeat has an identical structure, and that such repeats are connected tandemly to form a rod-like structure in solution, the length of which increased proportionately with the number of repeating units. On the basis of these results, it was proposed that Ebh is a 320 nm rod-like molecule with some plasticity at module junctions.

Original languageEnglish
Pages (from-to)488-496
Number of pages9
JournalStructure
Volume16
Issue number3
DOIs
Publication statusPublished - 2008 Mar 11
Externally publishedYes

Keywords

  • CELLBIO
  • MICROBIO

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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