A glass capillary microelectrode based on capillarity and its application to the detection of L-glutamate release from mouse brain slices

Kumiko Nakajima, Takashi Yamagiwa, Ayumi Hirano, Masao Sugawara

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

A new glass capillary microelectrode for L-glutamate is described using pulled glass capillaries (tip size, ∼12.5 μm) with a very small volume (-2 μl) of inner solution containing glutamate oxidase (GluOx) and ascorbate oxidase. The operation of the electrode is based on capillary action that samples L-glutamate into the inner solution. The enzyme reaction by GluOx generates hydrogen peroxide that is detected at an Os-gel-HRP polymer modified Pt electrode in a three-electrode configuration. The amperometric response behavior of the electrode was characterized in terms of the capillarity, response time, sensitivity and selectivity for measurements of L-glutamate. The currents at 0 V vs. Ag/AgCl increased linearly with the L-glutamate concentration from 10 to 150 μM for in vitro and in situ calibrations. The response was highly selective to L-glutamate over ascorbate, dopamine, serotonin and other amino acids. The detection of L-glutamate in the extracellular fluids of different regions of mouse hippocampal slices under stimulation of KCl was demonstrated.

Original languageEnglish
Pages (from-to)55-60
Number of pages6
Journalanalytical sciences
Volume19
Issue number1
DOIs
Publication statusPublished - 2003 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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