A genetic linkage map of the model legume Lotus japonicus and strategies for fast mapping of new loci

Niels Sandal, Lene Krusell, Simona Radutoiu, Magdalena Olbryt, Andrea Pedrosa, Silke Stracke, Shusei Sato, Tomohiko Kato, Satoshi Tabata, Martin Parniske, Andreas Bachmair, Tina Ketelsen, Jens Stougaard

Research output: Contribution to journalArticlepeer-review

60 Citations (Scopus)

Abstract

A genetic map for the model legume Lotusjaponicus has been developed. The F2 mapping population was established from an interspecific cross between L. japonicus and L. filicaulis. A high level of DNA polymorphism between these parents was the source of markers for linkage analysis and the map is based on a framework of amplified fragment length polymorphism (AFLP) markers. Additional markers were generated by restriction fragment length polymorphism (RFLP) and sequence-specific PCR. A total of 524 AFLP markers, 3 RAPD markers, 39 gene-specific markers, 33 microsatellite markers, and six recessive symbiotic mutant loci were mapped. This genetic map consists of six linkage groups corresponding to the six chromosomes in L. japonicus. Fluorescent in situ hybridization (FISH) with selected markers aligned the linkage groups to chromosomes as described in the accompanying article by PEDROSA et al. (2002, this issue). The length of the linkage map is 367 cM and the average marker distance is 0.6 cM. Distorted segregation of markers was found in certain sections of the map and linkage group I could be assembled only by combining colormapping and cytogenetics (FISH). A fast method to position genetic loci employing three AFLP primer combinations yielding 89 markers was developed and evaluated by mapping three symbiotic loci, Ljsym1, Ljsym5, and Ljhar1-3.

Original languageEnglish
Pages (from-to)1673-1683
Number of pages11
JournalGenetics
Volume161
Issue number4
Publication statusPublished - 2002 Aug 1
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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