TY - JOUR
T1 - A gene for a class II DNA photolyase from Oryza sativa
T2 - Cloning of the cDNA by dilution-amplification
AU - Hirouchi, T.
AU - Nakajima, S.
AU - Najrana, T.
AU - Tanaka, M.
AU - Matsunaga, T.
AU - Hidema, J.
AU - Teranishi, M.
AU - Fujino, T.
AU - Kumagai, T.
AU - Yamamoto, K.
N1 - Funding Information:
Acknowledgments This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan (13876073 to K.Y., 14704060 to J.H., and 12480154 to T.K.), by Grants for Research Proposals from the Research Institute of Innovative Technology for the Earth, and by a collaborative study of the Institute for Genetic Ecology, Tohoku University. T.N. is the recipient of a postdoctoral fellowship for foreign researchers from the Japan Society for the Promotion of Science (P-01345).
PY - 2003/7/1
Y1 - 2003/7/1
N2 - Ultraviolet radiation induces the formation of two classes of photoproducts in DNA - the cyclobutane pyrimidine dimer (CPD) and the pyrimidine [6-4] pyrimidone photoproduct (6-4 product). Many organisms produce enzymes, termed photolyases, which specifically bind to these lesions and split them via a UV-A/blue light-dependent mechanism, thereby reversing the damage. These photolyases are specific for either CPDs or 6-4 products. Two classes of photolyases (class I and class II) repair CPDs. A gene that encodes a protein with class II CPD photolyase activity in vitro has been cloned from several plants including Arabidopsis thaliana, Cucumis sativus and Chlamydomonas reinhardtii. We report here the isolation of a homolog of this gene from rice (Oryza sativa), which was cloned on the basis of sequence similarity and PCR-based dilution-amplification. The cDNA comprises a very GC-rich (75%) 5′ region, while the 3′ portion has a GC content of 50%. This gene encodes a protein with CPD photolyase activity when expressed in E. coli. The CPD photolyase gene encodes at least two types of mRNA, formed by alternative splicing of exon 5. One of the mRNAs encodes an ORF for 506 amino acid residues, while the other is predicted to code for 364 amino acid residues. The two RNAs occur in about equal amounts in O. sativa cells.
AB - Ultraviolet radiation induces the formation of two classes of photoproducts in DNA - the cyclobutane pyrimidine dimer (CPD) and the pyrimidine [6-4] pyrimidone photoproduct (6-4 product). Many organisms produce enzymes, termed photolyases, which specifically bind to these lesions and split them via a UV-A/blue light-dependent mechanism, thereby reversing the damage. These photolyases are specific for either CPDs or 6-4 products. Two classes of photolyases (class I and class II) repair CPDs. A gene that encodes a protein with class II CPD photolyase activity in vitro has been cloned from several plants including Arabidopsis thaliana, Cucumis sativus and Chlamydomonas reinhardtii. We report here the isolation of a homolog of this gene from rice (Oryza sativa), which was cloned on the basis of sequence similarity and PCR-based dilution-amplification. The cDNA comprises a very GC-rich (75%) 5′ region, while the 3′ portion has a GC content of 50%. This gene encodes a protein with CPD photolyase activity when expressed in E. coli. The CPD photolyase gene encodes at least two types of mRNA, formed by alternative splicing of exon 5. One of the mRNAs encodes an ORF for 506 amino acid residues, while the other is predicted to code for 364 amino acid residues. The two RNAs occur in about equal amounts in O. sativa cells.
KW - Alternative splicing
KW - GC content
KW - Oryza sativa
KW - Photolyase
KW - Solar UV radiation
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U2 - 10.1007/s00438-003-0856-9
DO - 10.1007/s00438-003-0856-9
M3 - Article
C2 - 12764611
AN - SCOPUS:0043124412
VL - 269
SP - 508
EP - 516
JO - Zeitschrift für Induktive Abstammungs- und Vererbungslehre
JF - Zeitschrift für Induktive Abstammungs- und Vererbungslehre
SN - 1617-4615
IS - 4
ER -